Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.0K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.0K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

193
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
193
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

4.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of sleep quality and its influencing factors among residents in Chengguan District of Lhasa.

BMC public health·2026
Same author

The oral nucleoside analogue inhibitor VV251 effectively inhibits coinfection by respiratory syncytial virus and influenza A virus.

Journal of virology·2026
Same author

Corrigendum to"A noval strategy of deletion in PK gene for construction of a vaccine candidate with exellent safety and complete protection efficiency against high virulent Chinese pseudorabies virus variant" [Virus Research 313 (2022) 198740].

Virus research·2026
Same author

From observation to protection a bibliometric analysis of aerosol-glacier interactions.

iScience·2026
Same author

K<sup>+</sup>/Cu<sup>2+</sup> Co-chelated diastereoselective Friedel-Crafts reaction with chiral <i>N</i>-sulfinyl ketimines for the facile synthesis of chiral bisindoles and their cytotoxicity.

RSC advances·2026
Same author

Enhanced carbon-nitrogen coupling and reduced organic carbon stability in the Liaohe River estuary wetland, China, induced by human activity.

Environmental research·2026
Same journal

Corrigendum to "Discovery of Benfotiamine as a subnanomolar P2Y<sub>14</sub>R antagonist for inflammatory diseases via drug repurposing and molecular dynamics-guided mechanism elucidation" [Eur. J. Med. Chem. 316 (2026) 119068].

European journal of medicinal chemistry·2026
Same journal

Identification of 3-arylquinoxalin-2(1H)-one derivatives targeting BRD4 BD1 as efficient anticancer agents.

European journal of medicinal chemistry·2026
Same journal

Discovery of novel ROCK inhibitors RX-021 and RX-044 with intraocular pressure-lowering effect for glaucoma treatment.

European journal of medicinal chemistry·2026
Same journal

Discovery of a novel benzoselenophene derivative as a potential therapeutic agent for pulmonary fibrosis.

European journal of medicinal chemistry·2026
Same journal

Design, synthesis and evaluation of dual modulators targeting glutaminyl cyclase and cannabinoid CB2 receptor.

European journal of medicinal chemistry·2026
Same journal

Discovery of N-(6-Acetylpyridin-3-yl)-2-(4-(ethylsulfonyl)phenyl)acetamide derivatives as a novel series of selective RORγt antagonists.

European journal of medicinal chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2025

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.3K

PROTAC technology: From drug development to probe technology for target deconvolution.

Si Yan1, Guangshuai Zhang1, Wei Luo2

  • 1National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology, Research Center for Precision Medication of Chinese Medicine, FuRong Laboratory, Hunan University of Chinese Medicine, Changsha, PR China; Hubei Engineering Research Center of Traditional Chinese Medicine of South Hubei Province, School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning, PR China; Department of Hepatology, China Military Institute of Chinese Materia, The Fifth Medical Center of PLA General Hospital, Beijing, PR China.

European Journal of Medicinal Chemistry
|July 31, 2024
PubMed
Summary
This summary is machine-generated.

Proteolysis-targeting chimera (PROTAC) technology offers a novel approach to drug development, effectively targeting previously intractable disease targets. PROTAC probes are revolutionizing drug discovery by enabling efficient target deconvolution and addressing challenges like drug resistance.

Keywords:
Drug developmentPROTACPROTAC probePro-PROTACTarget deconvolution

More Related Videos

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.3K
Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

4.5K

Related Experiment Videos

Last Updated: Jun 18, 2025

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.3K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.3K
Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

4.5K

Area of Science:

  • Pharmaceutical Science
  • Molecular Biology
  • Drug Discovery

Background:

  • Over 80% of disease targets are challenging to address with traditional drug development methods.
  • Proteolysis-targeting chimera (PROTAC) technology has emerged as a powerful tool to overcome these limitations.
  • PROTACs have evolved from peptide-based to small molecule E3 ligase ligands, with several candidates in clinical trials.

Purpose of the Study:

  • To provide a comprehensive overview of PROTAC technology's advancements in drug development and target discovery.
  • To systematically review the workflow of PROTAC probes.
  • To highlight the potential of PROTACs in addressing drug resistance, recurrence, and intractable targets.

Main Methods:

  • Review of existing literature on PROTAC technology and its applications.
  • Analysis of PROTAC probe design and workflow.
  • Examination of PROTACs' role in target deconvolution and drug discovery.

Main Results:

  • PROTAC technology has shown significant promise in overcoming drug resistance and targeting previously undruggable targets.
  • PROTAC probes offer advantages over traditional methods, especially for low-abundance or difficult-to-target proteins.
  • PROTACs are increasingly utilized for innovative drug target discovery and deconvolution.

Conclusions:

  • PROTAC technology represents a significant breakthrough in pharmaceutical research and development.
  • PROTAC probes are highly effective for target deconvolution due to their specificity and catalytic action.
  • PROTAC probe technology is expected to be a pivotal area in future drug target deconvolution research.