5-Fluorouracil-Loaded PLGA Declined Expression of Pro-Inflammatory Genes IL-9, IL-17A, IL-23 and IFN- y; in the HT-29 Colon Cancer Cell Line
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View abstract on PubMed
Summary
This summary is machine-generated.Encapsulating 5-fluorouracil (5-FU) into PLGA nanoparticles significantly reduced pro-inflammatory gene expression in HT-29 cells. This PLGA-5-FU nanoparticle formulation enhanced 5-FU
Area Of Science
- Nanotechnology in drug delivery
- Cancer biology and therapeutics
- Molecular oncology
Background
- Pro-inflammatory cytokines are crucial in cancer development and are targets for therapy.
- Understanding how cancer drugs like 5-fluorouracil (5-FU) affect cytokine expression can lead to new combination therapies.
- Investigating the impact of 5-FU encapsulated in PLGA nanoparticles on specific pro-inflammatory genes is key.
Purpose Of The Study
- To create and characterize Poly(lactic-co-glycolic acid)-5-fluorouracil (PLGA-5-FU) nanoparticles.
- To evaluate the effect of these nanoparticles on the expression of pro-inflammatory genes (IL-9, IL-17A, IL-23, IFN-γ) in HT-29 colon cancer cells.
- To compare the efficacy of encapsulated 5-FU with free 5-FU.
Main Methods
- PLGA-5-FU nanoparticles were synthesized and characterized using Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM).
- Cytotoxicity was assessed via MTT assay to determine the IC50.
- HT-29 cells were treated with varying concentrations of PLGA-5-FU nanoparticles, and gene expression was analyzed using quantitative Real-Time Polymerase Chain Reaction (qRT-PCR).
Main Results
- PLGA-5-FU nanoparticles were spherical with a mean size of 215.9 ± 43.3 nm and a negative charge.
- The nanoparticles exhibited dose- and time-dependent cytotoxicity against HT-29 cells.
- A significant, dose-dependent decrease in IL-9, IL-17A, IL-23, and IFN-γ gene expression was observed with PLGA-5-FU nanoparticles, unlike free 5-FU.
Conclusions
- PLGA-5-FU nanoparticles effectively suppressed the expression of key pro-inflammatory genes (IL-9, IL-17A, IL-23, IFN-γ) in HT-29 cells.
- Encapsulating 5-FU within PLGA nanoparticles significantly enhanced its therapeutic impact compared to free 5-FU.
- This formulation holds promise for improved cancer therapy by modulating the tumor microenvironment.
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