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Enhancing genomic prediction with Stacking Ensemble Learning in Arabica Coffee.

Moyses Nascimento1,2, Ana Carolina Campana Nascimento1,2, Camila Ferreira Azevedo1

  • 1Laboratory of Intelligence Computational and Statistical Learning (LICAE), Department of Statistics, Federal University of Viçosa, Viçosa, Brazil.

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Summary

Stacking Ensemble Learning (SEL) significantly improves genomic selection accuracy in Coffea Arabica breeding. This DNA-based approach enhances prediction of key traits like yield and disease resistance, outperforming traditional methods.

Keywords:
GBLUPensemble methodsplant breedingprediction accuracystatistical and machine learning

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Area of Science:

  • Plant Breeding and Genetics
  • Bioinformatics and Computational Biology
  • Agricultural Science

Background:

  • Traditional coffee breeding relies on lengthy phenotypic observations, hindering rapid cultivar development.
  • Genomic selection (GS) offers a faster, DNA-based alternative for identifying superior coffee (Coffea Arabica) genotypes.
  • Ensemble learning methods, particularly Stacking Ensemble Learning (SEL), show potential for enhancing prediction accuracy in complex trait selection.

Purpose of the Study:

  • To investigate the efficacy of Stacking Ensemble Learning (SEL) for improving prediction accuracy in Coffea Arabica genomic selection.
  • To evaluate SEL's performance in predicting key agronomic and disease resistance traits: yield (YL), fruit number (NF), leaf miner infestation (LM), and cercosporiosis incidence (Cer).

Main Methods:

  • Analysis of 195 Coffea Arabica individuals genotyped with 21,211 single-nucleotide polymorphism (SNP) markers.
  • Implementation of a cross-validation (CV) scheme to assess model performance.
  • Utilized Genomic Best Linear Unbiased Prediction (GBLUP), MARS, QRF, and RF as base learners within the SEL framework, with Ridge Regression, RF, GBLUP, and Single Average as meta-learners.

Main Results:

  • Stacking Ensemble Learning (SEL) demonstrated superior predictive ability (PA) across all evaluated traits compared to individual base learner models.
  • SEL achieved significant gains in PA over GBLUP: 87.44% for yield (YL), 37.83% for fruit number (NF), 199.82% for leaf miner infestation (LM), and 14.59% for cercosporiosis incidence (Cer).
  • The study confirmed SEL's capability to accurately predict important traits in Coffea Arabica.

Conclusions:

  • Stacking Ensemble Learning (SEL) represents a highly promising advancement for genomic selection in coffee breeding.
  • By integrating predictions from multiple models, SEL effectively enhances the predictive accuracy for complex traits in Coffea Arabica.
  • This approach accelerates the selection of superior coffee cultivars, addressing limitations of traditional breeding methods.