[A new strategy for malignancies by direct hemoperfusion using bacterial endotoxin bounded fiber]

Area of Science:

• Biomedical Engineering
• Immunology
• Oncology

Background:

• Lipopolysaccharide (LPS) from E. coli can be immobilized onto polystyrene fiber (LPS-F).
• Direct hemoperfusion (DHP) is a blood purification technique.
• Assessing the anti-cancer efficacy of LPS-F via DHP is crucial.

Purpose of the Study:

• To evaluate the anti-cancer activities of LPS-F using DHP in VX2 tumor-bearing rabbits.
• To investigate the impact of DHP on tumor growth and survival rates.
• To explore the potential of this method in conjunction with BCG immunotherapy.

Main Methods:

• VX2 tumor cells were implanted in rabbits.
• Direct hemoperfusion with LPS-F was performed at different stages post-tumor implantation.
• Rabbits were also infected with BCG intravenously before tumor implantation and DHP treatment.
• Tumor growth, survival rates, and histological changes were analyzed.

Main Results:

• DHP using LPS-F significantly suppressed tumor growth in both experimental groups (p < 0.05).
• Survival rates were improved in rabbits treated with DHP compared to control groups.
• Histological examination revealed tumor cell necrosis and bleeding at the tumor site post-DHP.
• In BCG-infected rabbits, DHP induced tumor necrosis and suggested the production of tumoricidal humoral factors.

Conclusions:

• Direct hemoperfusion with LPS-immobilized fiber exhibits direct tumoricidal activity against VX2 tumors in rabbits.
• This therapeutic approach shows promise for cancer treatment, potentially enhancing outcomes when combined with immunotherapy like BCG.