Analysis of metagenome and metabolome disclosed the mechanisms of Dendrobium officinale polysaccharide on DSS-induced ulcerative colitis-affected mice

Xiaona Zeng ¹, Shengqiu Tang ², Xiaoying Dong ²

¹Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, PR China; State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China.
²Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, PR China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, PR China.
³State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China.
⁴Division of Life Sciences and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China.

⁰Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, PR China; State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China.

International Journal of Biological Macromolecules +

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August 1, 2024

View abstract on PubMed

Summary

Dendrobium officinale polysaccharide (DOP) shows promise in treating ulcerative colitis (UC). This study found DOP improved gut health, reduced inflammation, and enhanced the intestinal barrier in mice with acute UC.

Area Of Science

Gastroenterology
Immunology
Pharmacology

Background

Ulcerative colitis (UC) is a challenging inflammatory bowel disease with unknown etiology.
Current treatments for UC have limitations, necessitating the exploration of novel therapeutic agents.

Purpose Of The Study

To investigate the protective effects of Dendrobium officinale polysaccharide (DOP) on acute ulcerative colitis (UC) induced by dextran sulphate sodium (DSS) in a mouse model.
To elucidate the underlying mechanisms of DOP's therapeutic action in UC.

Main Methods

Acute UC was induced in mice using DSS.
Mice were treated with DOP, and various parameters including disease activity index (DAI), inflammatory cytokine levels (IL-2, IL-4, IL-6, IL-10), intestinal barrier integrity (goblet cell density, tight junctions), oxidative stress markers (T-AOC, GSH, NO, MDA), serum biochemistry, and gut microbiota composition (Lactobacillales) were assessed.

Main Results

DOP treatment significantly ameliorated DSS-induced UC symptoms, including reduced weight loss and DAI.
DOP improved intestinal barrier function by increasing goblet cell density and maintaining tight junctions.
DOP modulated inflammatory cytokine release, enhanced total antioxidant capacity, and altered serum biochemical profiles.
DOP treatment increased the abundance of Lactobacillales and regulated metabolic pathways involved in anti-inflammatory and antiviral substance synthesis.

Conclusions

Dendrobium officinale polysaccharide (DOP) demonstrates significant protective effects against DSS-induced acute ulcerative colitis (UC) in mice.
DOP exerts its therapeutic benefits by preserving intestinal barrier integrity, reducing oxidative stress, modulating the immune response, and influencing gut microbiota.
DOP holds potential as a functional dietary supplement for managing UC.

Keywords:

Dendrobium officinale polysaccharide Metabolome Metagenome Ulcerative colitis (UC)