Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
7.1K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

14.9K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
14.9K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
What is Population Genetics?01:25

What is Population Genetics?

57.8K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
57.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exercise reprograms the gut microbiota to enhance metabolic outcomes after bariatric surgery: a translational, cross-species study.

International journal of obesity (2005)·2026
Same author

Epigenetic blind spots - the role of DNA methylation dynamics in stem cell-based models of embryogenesis.

FEBS letters·2026
Same author

The Niebuhr net: A net for capturing benthopelagic fish and fauna with towed camera systems.

HardwareX·2026
Same author

Host transcriptomic analysis reveals a defective intracellular environment that limits SARS-CoV-2 replication in CFTR-deficient airway epithelium.

Frontiers in cellular and infection microbiology·2026
Same author

Comparative Multi-Marker Environmental DNA Metabarcoding of Marine Metazoan Communities: Water vs. Sediment.

Molecular ecology resources·2026
Same author

Chromosome-level genome of the Adriatic sturgeon, Acipenser naccarii: A resource for polyploid fish genomics.

The Journal of heredity·2026

Related Experiment Video

Updated: Jun 18, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.1K

MIPs: multi-locus intron polymorphisms in species identification and population genomics.

Elisa Boscari1, Stefano Dalle Palle2, Nicola Vitulo3

  • 1Department of Biology, University of Padova, Via Ugo Bassi 58B, 35121, Padova, Italy. elisa.boscari@unipd.it.

Scientific Reports
|August 1, 2024
PubMed
Summary

We developed multi-locus intron polymorphisms (MIPs) to analyze genetic diversity and hybridization. This new method uses intron regions as versatile markers, applicable across species without prior genetic knowledge.

Keywords:
Forensic identificationHigh-throughput DNA sequencingMolecular markersMultiple-SNP haplotypesNon-model organismsTeleost fishes

More Related Videos

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.1K
Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster
04:59

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

Published on: March 28, 2025

518

Related Experiment Videos

Last Updated: Jun 18, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.1K
Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.1K
Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster
04:59

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

Published on: March 28, 2025

518

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Analyzing species groups with hybridization or introgression requires shared molecular markers.
  • Current methods use microsatellites, SNPs, or microhaplotypes via high-throughput sequencing.

Purpose of the Study:

  • To develop and validate a novel method using intron-targeted amplicon sequencing for genetic analysis.
  • To create a transferable panel of multi-locus intron polymorphisms (MIPs) for diverse applications.

Main Methods:

  • Utilized intron-targeted amplicon sequencing to characterize multi-locus intron polymorphisms (MIPs).
  • Developed a panel of MIPs transferable across fish genomes.
  • Assessed genetic diversity and population structure.

Main Results:

  • The MIPs panel effectively distinguished between closely related fish species and populations.
  • MIPs demonstrated high variability and transferability across different fish genomes.
  • The method proved useful for identifying species, hybrids, and population structures.

Conclusions:

  • MIPs are versatile, hypervariable nuclear markers suitable for various genetic applications, including hybridization monitoring.
  • The intron-targeted sequencing approach unlocks a new source of genetic variation.
  • This method, demonstrated in fish, is readily applicable to other taxa.