Novel mixed self-assembled monolayers of L-cysteine and methanol on gold surfaces under ambient conditions

In this work, we carried out an experimental and theoretical study on the formation of self-assembled monolayers of L-cysteine molecules on gold surfaces in the presence of methanol as a solvent. We report for the first time L-cysteine and methanol ordered structures forming a mixed self-assembled mono-layer on Au(100) surfaces under ambient conditions. Finger-like ordered structures with a relative height of 0.10-0.20 nm, average width of 2.0 nm and variable lengths were observed using scanning tunneling microscopy under room temperature and ambient pressure conditions. Using X-ray photoemission spectroscopy, it was determined that L-cysteine molecules bind to the gold surface through the sulfur atom of their thiol group in two molecular configurations: neutral and zwitterionic. We found that the finger-like structures are the result of complex interactions of L-cysteine molecules with gold surfaces and L-cysteine molecules with methanol molecules and among all three components of the system (L-cysteine + methanol + gold surfaces). These interactions were detected through attenuated total reflectance-Fourier transform infrared spectroscopy. Furthermore, adsorbate/substrate interactions were studied by employing ab initio calculations using density functional theory, resulting in molecular arrangements formed by chains of L-cysteine pairs surrounded by physisorbed methanol molecules.