Cell cycle-driven transcriptome maturation confers multilineage competence to cardiopharyngeal progenitors
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View abstract on PubMed
Summary
This summary is machine-generated.Cellular development involves precise timing. This study reveals how cardiopharyngeal progenitors gain specific cell fates by coordinating gene expression changes with cell division cycles, ensuring proper tissue formation.
Area Of Science
- Developmental Biology
- Cellular and Molecular Biology
- Genomics
Background
- Stem and progenitor cells differentiate into diverse cell types during development.
- This process involves changes in gene expression and cell potency.
- Multipotent progenitors often exhibit transcriptional priming for multiple lineages.
Purpose Of The Study
- To investigate how multipotent progenitors acquire multilineage competence.
- To understand the coupling between cell fate decisions and the cell cycle.
- To explore transcriptome dynamics during cell cycle progression in cardiopharyngeal progenitors.
Main Methods
- Utilized the tunicate Ciona as a model organism.
- Combined transgene-based sample barcoding with single-cell RNA sequencing (scRNA-seq).
- Performed functional assays to assess gene function and cell behavior.
Main Results
- Identified that cardiopharyngeal progenitors gain competence before mitosis, linked to Tbx1/10 activation.
- Uncovered "transcriptome maturation" dynamics across cell cycle phases (G1, S, G2).
- Found that "mature genes" like Depdc1 peak in late G2, influencing cell division and fate.
Conclusions
- Transcriptome maturation, driven by feedforward circuits and cell cycle coupling, promotes "behavioral competence".
- Coupling cell cycle phases (G2 for competence, G1 for fate) ensures timely lineage program deployment.
- This study reveals intricate coordination between cell cycle progression and developmental fate decisions.
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