Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K
X-ray Crystallography02:18

X-ray Crystallography

23.8K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
23.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Report of high data rate macromolecular crystallography (HDRMX) meeting, 23 July 2025.

Structural dynamics (Melville, N.Y.)·2026
Same author

Simulating neutron protein crystallography experiments: applications to the development of the NMX instrument at ESS.

Acta crystallographica. Section D, Structural biology·2026
Same author

Chaperonin recognition of protein dynamics drives drug resistance.

bioRxiv : the preprint server for biology·2026
Same author

Inferring structure factors of weakly populated excited states in perturbative crystallography experiments.

bioRxiv : the preprint server for biology·2026
Same author

AlphaFold as a prior: experimental structure determination conditioned on a pretrained neural network.

Nature methods·2026
Same author

Stabilizing Structural Transitional States between 1- and 2-Dimensional Topologies via Hydrogen Bond-Mediated Crystal Engineering.

Journal of the American Chemical Society·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2025

High Pressure Single Crystal Diffraction at PX^2
11:32

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

21.5K

Laue-DIALS: open-source software for polychromatic X-ray diffraction data.

Rick A Hewitt1, Kevin M Dalton2,3,1, Derek Mendez4

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138.

Biorxiv : the Preprint Server for Biology
|August 2, 2024
PubMed
Summary
This summary is machine-generated.

New software, Laue-DIALS, enables efficient analysis of polychromatic X-ray diffraction data. This advancement allows researchers to utilize more X-ray photons, improving time-resolved experiments and reducing data loss.

More Related Videos

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.0K
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

29.9K

Related Experiment Videos

Last Updated: Jun 18, 2025

High Pressure Single Crystal Diffraction at PX^2
11:32

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

21.5K
Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

9.0K
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

29.9K

Area of Science:

  • Crystallography and Materials Science
  • X-ray Diffraction Analysis
  • Scientific Software Development

Background:

  • X-ray diffraction experiments often utilize polychromatic (pink) X-rays for efficiency and enhanced data collection, particularly for time-resolved studies.
  • However, analyzing polychromatic X-ray diffraction data presents significant challenges, leading most facilities to process only monochromatic data and discard valuable photon information.
  • Existing challenges include accurate lattice searching, indexing, wavelength assignment, and correcting for wavelength-dependent intensity variations and harmonic deconvolution.

Purpose of the Study:

  • To introduce Laue-DIALS, an open-source software pipeline designed for indexing and integrating polychromatic X-ray diffraction data.
  • To demonstrate the combined utility of Laue-DIALS with the Careless algorithm for comprehensive polychromatic data analysis.
  • To provide a robust and extensible platform for advancing polychromatic X-ray diffraction studies, especially in time-resolved applications.

Main Methods:

  • Development of Laue-DIALS, an open-source software pipeline built upon the dxtbx toolbox, which integrates with the established DIALS software.
  • Integration of Laue-DIALS with the Careless algorithm for performing harmonic deconvolution and correcting measured intensities for wavelength variations.
  • Benchmarking of the Laue-DIALS and Careless pipeline using polychromatic diffraction data to assess its performance and suitability.

Main Results:

  • Laue-DIALS successfully indexes and integrates polychromatic X-ray diffraction data, overcoming previous analytical limitations.
  • The combined use of Laue-DIALS and Careless provides a viable method for analyzing polychromatic diffraction data, including correction for wavelength-dependent effects.
  • Benchmark results confirm the effectiveness of the proposed software pipeline for processing complex polychromatic diffraction datasets.

Conclusions:

  • Laue-DIALS, in conjunction with Careless, offers a powerful and open-source solution for the comprehensive analysis of polychromatic X-ray diffraction data.
  • This approach enables the efficient utilization of polychromatic X-rays, significantly improving data collection and analysis for time-resolved crystallographic applications.
  • The modular and extensible architecture of Laue-DIALS ensures its adaptability for future advancements in X-ray diffraction data processing.