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Published on: June 7, 2019
A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo
Takaki Yahiro1, Landon Bayless-Edwards1, James A Jones1
1Vollum Institute, Oregon Health and Science University, Portland, OR, 97239.
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We report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Förster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enables in vivo imaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.
