Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estrogen Stabilizes Interferon-Inducible Genes During Skeletal Muscle Regeneration.

Biological research for nursing·2025
Same author

Proteomic and <i>N</i>-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes <i>in vitro</i>.

Microbiology spectrum·2025
Same author

Notch Is Required for Neural Progenitor Proliferation During Embryonic Eye Regrowth.

International journal of molecular sciences·2025
Same author

Proteomic and <i>N</i>-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes.

bioRxiv : the preprint server for biology·2024
Same author

Temporal Transcriptomic Profiling of the Developing <i>Xenopus laevis</i> Eye.

Cells·2024
Same author

Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in <i>Manduca sexta</i>.

Insects·2024
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2025

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
06:32

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

6.2K

Temporal Transcriptomic Profiling of the Developing Xenopus laevis Eye.

Samantha J Hack1, Juli Petereit2, Kelly Ai-Sun Tseng3

  • 1Department of Biological Sciences, Western Michigan University, Kalamazoo, MI 49008, USA.

Biorxiv : the Preprint Server for Biology
|August 2, 2024
PubMed
Summary
This summary is machine-generated.

This study maps the molecular signature of retinal progenitor cells (RPCs) during development and regeneration in Xenopus. Understanding RPC gene expression is crucial for developing treatments for visual impairments.

Keywords:
NotchXenopuscell signalingeye developmentocular transcriptomeretinaretinal progenitor cells

More Related Videos

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

2.8K
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
10:22

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Published on: December 23, 2012

13.8K

Related Experiment Videos

Last Updated: Jun 18, 2025

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
06:32

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

6.2K
Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

2.8K
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
10:22

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Published on: December 23, 2012

13.8K

Area of Science:

  • Developmental Biology
  • Genomics
  • Ophthalmology

Background:

  • Retinal progenitor cells (RPCs) generate all retinal cell types.
  • Understanding RPC molecular signatures is key for treating visual impairments.

Purpose of the Study:

  • Define the transcriptomic profiles of RPCs during Xenopus retinogenesis.
  • Identify molecular regulators of RPC proliferation and differentiation.

Main Methods:

  • RNA-sequencing of whole Xenopus eyes at three embryonic stages.
  • Differential gene expression analysis.
  • Gene Ontology and KEGG pathway analyses.

Main Results:

  • Upregulation of developmental pathways (Wnt, Hedgehog) during RPC proliferation (st. 24, 27).
  • Shift to metabolic and phototransduction pathways during RPC differentiation (st. 35).
  • Expression of conserved eye regeneration genes (sox2, pax6, nrl, Notch signaling) during development.

Conclusions:

  • The study provides a comprehensive transcriptomic resource for RPCs during retinogenesis.
  • Identified key developmental and regeneration genes involved in RPC activity.
  • Dataset facilitates future research into eye repair and regeneration mechanisms.