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Related Experiment Video

Updated: Jun 18, 2025

In Vitro Model of Coronary Angiogenesis

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

Intramyocardial Sprouting Tip Cells Specify Coronary Arterialization.

Elena Cano^1,2,3,4,5, Jennifer Schwarzkopf^1,2,3, Masatoshi Kanda^6,7

¹Integrative Vascular Biology Laboratory (E.C., J.S., I.H., H.G.), Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.

Circulation Research

|August 2, 2024

Summary

This summary is machine-generated.

Coronary arteries develop from tip cells, a process crucial for heart vascularization and repair. This tip cell-to-artery pathway is conserved from development to adulthood and reactivated in ischemic heart conditions.

Keywords:

angiogenesis arteries coronary vessels endocardium endothelium

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Area of Science:

Cardiovascular Biology
Developmental Biology
Endothelial Cell Biology

Background:

Coronary artery patterning is vital for heart function but poorly understood.
Mechanisms of endothelial cell coordination and arteriovenous specification are unknown.
Knowledge is crucial for therapies targeting ischemic heart disease.

Purpose of the Study:

To delineate transcriptional states of developing and mature coronary endothelium.
To investigate the origin and mechanisms of coronary angiogenic sprouting.
To assess conservation of endothelial cell states across development, disease, and species.

Main Methods:

Single-cell transcriptomics and histological validation.
Genetic lineage tracing and 3D imaging.
Integration of transcriptomic data from mouse and human embryonic hearts.

Main Results:

Coronary arteries originate from cells with a tip cell phenotype.
Distinct intramyocardial and subepicardial tip cell populations identified.
Tip cell-to-artery specification is a conserved mechanism, present from development to adulthood and in response to injury.

Conclusions:

A tip cell-to-artery specification mechanism drives coronary arterialization throughout life.
This mechanism is reactivated in the adult heart following ischemic injury.
Differential sprouting programs regulate arterial and venous coronary plexus formation.