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Lipids as Anchors

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...

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Updated: Jun 21, 2026

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

Protocol for evaluating S-acylated protein membrane affinity using protein-lipid conjugates.

Chunyang Xie¹, Dong Luo², Lu Liu¹

¹School of Life Sciences, Chongqing University, Chongqing 401331, China.

|August 2, 2024

Summary

This summary is machine-generated.

This study introduces a new in vitro method to assess how S-acylated proteins bind to membranes. The protocol details creating protein-lipid conjugates for evaluating membrane association of S-acylated proteins.

Keywords:

Cell Membrane Chemistry Protein expression and purification

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Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

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Published on: May 13, 2020

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

S-acylation is a post-translational modification crucial for protein localization and function.
S-acylated proteins often associate with cellular membranes, but evaluating this interaction in vitro can be challenging.

Purpose of the Study:

To establish a robust in vitro platform for evaluating the membrane affinity of S-acylated proteins.
To provide a detailed protocol for preparing necessary reagents and performing the membrane binding assay.

Main Methods:

Preparation of lipid-maleimide compounds and mCherry-p62 recombinant proteins.
Synthesis of protein-lipid conjugates using the prepared reagents.
Evaluation of the membrane affinity of the synthesized protein-lipid conjugates using cellular membranes.

Main Results:

Successful synthesis of protein-lipid conjugates.
Demonstration of the protocol's utility in assessing S-acylated protein-membrane interactions.
Establishment of a reproducible in vitro system for membrane association studies.

Conclusions:

The developed protocol provides a valuable tool for studying S-acylated protein-membrane interactions in vitro.
This platform facilitates research into the functional roles of S-acylation in protein localization and signaling.