Visualization of nonsmall-cell lung cancer by near-infrared fluorescence imaging with tumor-targeting peptide ABT-510

Yuanbiao Tu ¹, Minfang Gao ¹, Tianming Tao ¹

⁰Cancer Research Center, the Jiangxi Province Key Laboratory for Diagnosis, Treatment, and Rehabilitation of Cancer in Chinese Medicine, Jiangxi Engineering Research Center for Translational Cancer Technology, Jiangxi University of Chinese Medicine, Nanchang 330004, China.

Bioorganic Chemistry +

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August 2, 2024

View abstract on PubMed

Summary

A novel near-infrared fluorescent probe, MPA-ABT-510, targets CD36 for improved nonsmall-cell lung cancer (NSCLC) visualization. This probe shows high tumor accumulation and strong binding affinity, aiding in surgical navigation for NSCLC patients.

Area Of Science

Oncology
Medical Imaging
Molecular Biology

Background

Nonsmall-cell lung cancer (NSCLC) is the most common lung malignancy.
Accurate visualization of NSCLC is crucial for effective surgical treatment.
Current imaging methods face challenges due to lung anatomy and respiratory motion.

Purpose Of The Study

To investigate CD36 as a potential imaging target for NSCLC.
To develop and evaluate a novel near-infrared (NIR) fluorescent probe for NSCLC.
To assess the probe's efficacy in vitro and in vivo for NSCLC detection and surgical navigation.

Main Methods

Constructed a water-soluble NIR fluorescent probe, MPA-ABT-510, by conjugating ABT-510 with MPA.
Performed molecular docking to predict binding affinity to CD36.
Conducted in vitro cell binding assays using NSCLC cell lines (H1299, A549).
Evaluated in vivo tumor targeting and accumulation using subcutaneous and orthotopic mouse models.
Assessed biodistribution in liver and intestinal metastasis models.

Main Results

MPA-ABT-510 demonstrated strong binding affinity to CD36 via molecular docking.
In vitro assays confirmed specific binding of ABT-510 to CD36-high NSCLC cells.
In vivo imaging showed efficient accumulation of MPA-ABT-510 in NSCLC tumor sites.
High tumor-to-lung and tumor-to-liver fluorescence ratios were observed in metastasis models.
Significant accumulation was noted in intestinal metastasis models.

Conclusions

MPA-ABT-510 is a selective and effective fluorescent probe for NSCLC.
The probe exhibits excellent imaging capabilities with minimal safety concerns.
MPA-ABT-510 holds promise as a tool for NSCLC surgical navigation.

Keywords:

Intestinal metastases Liver metastasis Near-infrared fluorescence imaging Nonsmall-cell lung cancer Orthotopic tumour