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Related Experiment Video

Updated: Jun 18, 2025

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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Innovations Toward Immunopeptidomics.

Jennifer G Abelin1, Andrea L Cox2

  • 1Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Molecular & Cellular Proteomics : MCP
|August 2, 2024
PubMed
Summary
This summary is machine-generated.

Immunopeptidomics, driven by mass spectrometry, identifies T cell targets. Pioneering work by Professor Donald F. Hunt

Keywords:
HLAMHCantigenimmunopeptidomicspeptidomics

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Last Updated: Jun 18, 2025

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Area of Science:

  • Immunology
  • Biochemistry
  • Mass Spectrometry

Background:

  • Immunopeptidomics has advanced significantly over 30 years.
  • Improvements in mass spectrometry, genomics, and T cell receptor sequencing have been key.
  • Collaborative efforts in immunology and chemistry enabled MHC-bound peptide identification.

Observation:

  • Highly sensitive mass spectrometry was innovatively applied to study MHC-presented peptide antigens.
  • This approach continues to be refined to understand T cell antigen presentation.
  • Professor Donald F. Hunt's laboratory pioneered these mass spectrometry-based immunopeptidomics techniques.

Findings:

  • The Hunt laboratory applied immunopeptidomics to cancer, autoimmunity, transplant rejection, and infectious diseases.
  • They identified novel MHC peptide-binding motifs and immunologically active antigens.
  • Many MHC-bound peptides were sequenced via mass spectrometry even before the human genome was completed.

Implications:

  • This work forms a foundation for mass spectrometry-based immunopeptidomics.
  • It advances our understanding of T cell-mediated immune responses.
  • Applications span translational research in diverse immunological contexts.