Sensitive high performance liquid chromatographic analysis of ethmozin in plasma

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Ethmozin is an antiarrhythmic drug requiring precise quantification.
• Accurate measurement of ethmozin is crucial for therapeutic drug monitoring and pharmacokinetic studies.

Purpose of the Study:

• To develop and validate a sensitive high-performance liquid chromatography (HPLC) method for the quantitative determination of ethmozin.
• To establish a reliable analytical procedure for ethmozin in biological matrices.

Main Methods:

• A one-step liquid-liquid extraction using diethylether at pH 9.0.
• Separation on a CN-bonded column with a methanol-propanol-2-perchloric acid mobile phase.
• Detection of ethmozin at 268 nm using HPLC.

Main Results:

• The developed HPLC method demonstrated high sensitivity.
• The lower limit of detection (LOD) was 11 nM, and the lower limit of quantification (LOQ) was 22 nM (10 ng/ml).
• The method exhibited linearity over a wide concentration range (0–8,620 nM).

Conclusions:

• A sensitive and reliable HPLC method for ethmozin quantification has been established.
• This method is suitable for accurate determination of ethmozin in relevant concentrations.
• The procedure facilitates therapeutic drug monitoring and research involving ethmozin.