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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 18, 2025

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Best practices for user consultation in flow cytometry shared resource laboratories.

Kewal Asosingh¹, Alice Bayiyana², Michele C Black³

¹Cleveland Clinic Lerner Research Institute, Flow Cytometry SRL, Cleveland, United States.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|August 3, 2024

Summary

This summary is machine-generated.

Consulting users early in flow cytometry projects improves experimental design and data interpretation. This best practice enhances reproducibility and resource efficiency in shared research laboratories.

Keywords:

best practices consultation flow cytometry shared laboratory resource

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Last Updated: Jun 18, 2025

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Area of Science:

Cytometry
Biotechnology
Laboratory Management

Background:

Shared Research Laboratories (SRLs) are crucial for advanced research.
User consultation is vital for optimizing flow cytometry experiments.
Effective communication is key to successful research collaborations.

Purpose of the Study:

To outline best practices for user consultation in flow cytometry.
To provide a framework for productive communication between SRLs and users.
To enhance the quality and efficiency of flow cytometry research.

Main Methods:

Analysis of a 2022 International Society for the Advancement of Cytometry (ISAC) membership survey.
Incorporation of insights from a CYTO 2022 workshop discussion.
Development of guidelines for optimal user consultation.

Main Results:

Early consultation is critical, especially for trainees, to standardize experimental steps.
Proactive consultation minimizes bias and reduces trial-and-error, saving time and resources.
Defined communication channels and information exchange foster robust collaborations.

Conclusions:

Implementing these best practices standardizes flow cytometry workflows and improves research reproducibility.
User consultation empowers researchers with essential information for scientifically rigorous experiments.
SRLs can adapt these guidelines to formalize consultation services and enhance research outcomes.