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Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
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Visualizing DNA/RNA, Proteins, and Small Molecule Metabolites within Live Cells
Dongling Jia1, Minhui Cui1, Xianting Ding2
1School of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China.
Small (Weinheim an Der Bergstrasse, Germany)
|August 3, 2024
Summary
Live cell imaging advances offer critical insights into molecular dynamics for disease diagnosis and treatment. This review covers new techniques for tracking RNAs, DNAs, and proteins in living systems.
Area of Science:
- Molecular biology
- Cell biology
- Biomedical imaging
Background:
- Live cell imaging provides crucial spatial and temporal data on molecular events in cells and organisms.
- Tracking molecules in live cells is vital for understanding physiological processes in various biological fields.
Purpose of the Study:
- To provide a comprehensive overview of recent advancements in live-cell imaging techniques.
- To summarize applications of live-cell imaging in molecular, immuno-, and biochemical diagnostics.
- To discuss challenges and future prospects in the field of live-cell imaging.
Main Methods:
- Review of recent research progress in live-cell imaging.
- Summary of advanced imaging techniques: high-precision, high-resolution, low-abundance, multidimensional, multipath, rapid, and computational methods.
Main Results:
- Live-cell imaging enables tracking of RNAs, DNAs, proteins, and metabolites.
- Advanced techniques enhance imaging capabilities for dynamic molecular events.
- Applications span molecular diagnosis, immunodiagnosis, and biochemical diagnosis.
Conclusions:
- Live-cell imaging techniques offer valuable insights for disease prevention, diagnosis, and treatment.
- Continued development of imaging methods promises further breakthroughs in understanding and combating diseases.
