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A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies

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Hydrocortisone treatment as a tool to study conjunctival placode induction.

Paige M Drake¹, Tamara A Franz-Odendaal²

¹Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|August 3, 2024

Summary

Hydrocortisone treatment in embryonic chicken eyes inhibits conjunctival papillae development by reducing Fgf20 expression. This placode development study reveals insights into vertebrate eye bone formation.

Keywords:

FGF20 apoptosis induction ocular skeleton scleral ossicles vasculature

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Area of Science:

Developmental biology
Ophthalmology
Molecular biology

Background:

Conjunctival placodes are crucial for forming conjunctival papillae and scleral ossicles in vertebrate eyes.
This study investigates the impact of hydrocortisone on embryonic chicken eye development, specifically on conjunctival papillae.

Purpose of the Study:

To establish a hydrocortisone injection protocol to inhibit conjunctival papillae formation in embryonic chicken eyes.
To assess the effects of this treatment on apoptosis, vasculature, and gene expression related to placode development.

Main Methods:

Administered hydrocortisone via injection to embryonic chicken eyes.
Assessed apoptosis, ciliary artery and vascular plexus integrity.
Analyzed gene expression of β-catenin, Eda, and Fgf20.

Main Results:

Hydrocortisone treatment did not increase apoptosis or significantly alter vasculature.
β-catenin and Eda expression remained unchanged, despite the absence of conjunctival papillae.
Fgf20 expression was significantly reduced, and β-catenin distribution was altered by hydrocortisone.

Conclusions:

Conjunctival papillae induction starts by embryonic day 5.5 (HH27.5).
Hydrocortisone reduces Fgf20 expression, potentially impacting Wnt/β-catenin or Eda/Edar pathways.
Findings enhance understanding of vertebrate eye development and scleral ossicle formation.