Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Karyotyping

Karyotyping

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Novel Variant in an Israeli Bedouin Family: The First Reported Cases of Carbonic Anhydrase VA (CA5A) Deficiency in Israel.

Genes·2026

Same author

The Sol-Gel Metal-Oxide Skeleton Affects the Catalytic Properties of In Situ Formed Metal Nanoparticles.

ACS omega·2026

Same author

Bowel incision closure with a semi-mutomated Robot-assisted laser tissue soldering system.

Scientific reports·2026

Same author

[Monogenic Kidney Diseases in Children - Five Years of Experience from a Dedicated Nephrogenetic Clinic in Southern Israel].

Harefuah·2025

Same author

The response of rare CFTR mutations to specific modulator combinations.

ERJ open research·2025

Same author

Dip-Pen Nanolithography-Based Fabrication of Meta-Chemical Surface for Heavy Metal Detection: Role of Poly-Methyl Methacrylate in Sensor Sensitivity.

Small science·2025

Search research articles

Related Experiment Video

Updated: Jun 18, 2025

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Published on: February 6, 2012

Adaptive Segmentation of DAPI-stained, C-banded, Aggregated and Overlapping Chromosomes.

Max Platkov¹, Ziv J Gardos², Lena Gurevich³

¹Nuclear Research Center Negev, Beer-Sheba, 84190, Israel. mmaaxxpp@gmail.com.

Cell Biochemistry and Biophysics

|August 4, 2024

Summary

New adaptive algorithms improve automated segmentation of difficult metaphase chromosome spreads. These methods enhance chromosome and centromere detection in the Dicentrics Chromosome Assay (DCA), overcoming challenges like overlapping and aggregation.

More Related Videos

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation

Published on: September 4, 2017

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

Related Experiment Videos

Last Updated: Jun 18, 2025

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Published on: February 6, 2012

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification ADCI and Dose Estimation

Published on: September 4, 2017

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

Area of Science:

Cytogenetics
Computational Biology
Image Analysis

Background:

Automated chromosome and centromere segmentation algorithms struggle with condensed, C-banded, and DAPI-stained chromosomes.
Overlapping and aggregated chromosomes in metaphase spreads pose significant challenges for accurate counting in the Dicentrics Chromosome Assay (DCA).

Purpose of the Study:

To introduce adaptive algorithms for segmenting challenging metaphase spreads, specifically addressing overlapping and aggregated chromosomes.
To enhance the accuracy and reliability of chromosome and centromere detection in cytogenetic analysis.

Main Methods:

Developed adaptive algorithms with two key optimizations: automatic selection of the best algorithm and binary search optimization of parameters.
Employed skeleton junctions, object thickness reduction, and the watershed algorithm for segmenting chromosomal clusters.
Utilized rule-based characterization of chromosomes and cluster analysis with shape/intensity profiles for centromere detection.

Main Results:

Successfully segmented mildly to moderately aggregated chromosomal clusters.
Achieved high sensitivity and specificity in detecting both chromosomes and centromeres.
Demonstrated improved performance on difficult metaphase spreads compared to existing methods.

Conclusions:

The proposed adaptive algorithms effectively address limitations of current methods for chromosome and centromere segmentation.
These algorithms provide a robust solution for analyzing challenging metaphase spreads in cytogenetic studies, including the DCA.
The developed techniques enhance the precision of automated chromosome analysis and counting.