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Targeted saliva metabolomics in Sjögren's syndrome
Giovanna Piacenza Florezi1, Felippe Pereira Barone2, Mario Augusto Izidoro3
1Stomatology Department, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brazil; Tropical Medicine Institute, LIM-06, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
Objective:
Sjögren's Syndrome (SS) is a chronic inflammatory autoimmune exocrinopathy, and although, the role of metabolism in the autoimmune responses has been discussed in diseases such as lupus erythematosus, rheumatoid arthritis, psoriasis and scleroderma. There is a lack of information regarding the metabolic implications of SS. Considering that the disease affects primarily salivary glands; the aim of this study is to evaluate the metabolic changes in the salivary glands' microenvironment using a targeted metabolomics approach.
Methods:
The saliva from 10 patients diagnosed with SS by the American-European consensus and 10 healthy volunteers was analyzed in an Ultra-high Performance Liquid Chromatograph Coupled Mass Spectrometry (UPLC-MS).
Results:
The results showed an increased concentration in SS of metabolites involved in oxidative stress such as lactate, alanine and malate, and amino acids involved in the growth and proliferation of T-cells, such as arginine, leucine valine and isoleucine.
Conclusions:
These results revealed that is possible to differentiate the metabolic profile of SS and healthy individuals using a small amount of saliva, which in its turn may reflect the cellular changes observed in the microenvironments of damaged salivary glands from these patients.
