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Efficient Estimation of ascorbic acid in vitamin C tablets enabled by phase-transfer strategy.

Wei-Qi Xie1, Yi-Xan Gong1

  • 1School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.

Analytical Biochemistry
|August 4, 2024
PubMed
Summary

A new phase-transfer method efficiently detects ascorbic acid (vitamin C) in tablets. This approach uses headspace gas chromatography for precise and sensitive analysis, ideal for industrial batch testing.

Keywords:
Ascorbic acidHeadspacePhase-transfer strategyVitamin C tablets

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis

Background:

  • Ascorbic acid (vitamin C) is a vital nutrient, and its accurate quantification in supplements is crucial.
  • Traditional methods for ascorbic acid determination can be time-consuming and prone to errors, especially in batch analysis.

Purpose of the Study:

  • To develop and validate a novel, efficient phase-transfer strategy for the batch detection of ascorbic acid in vitamin C tablets.
  • To leverage headspace gas chromatography (GC) for sensitive and precise indirect quantification of ascorbic acid.

Main Methods:

  • A phase-transfer reaction involving ascorbic acid and potassium permanganate.
  • Quantification of residual potassium permanganate via reaction with sodium oxalate to produce carbon dioxide.
  • Measurement of generated carbon dioxide using headspace GC for indirect ascorbic acid determination.

Main Results:

  • The headspace GC method demonstrated high precision, with a relative standard deviation below 2.11%.
  • The method exhibited excellent sensitivity, achieving a limit of quantitation of 0.27 μmol.
  • The developed approach proved reliable for ascorbic acid determination in vitamin C tablets.

Conclusions:

  • The novel phase-transfer strategy combined with headspace GC offers an efficient, precise, and sensitive method for ascorbic acid analysis.
  • The automated nature of headspace GC enhances batch sample detection efficiency and minimizes human operational errors.
  • This method is highly suitable for large-scale industrial analysis of ascorbic acid in pharmaceutical products.