Protective effects of degraded Bletilla striata polysaccharides against UVB-induced oxidative stress in skin
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View abstract on PubMed
Summary
This summary is machine-generated.Degraded Bletilla striata polysaccharides (DBSPs) show improved antioxidant and anti-photoaging effects. DBSP-5 effectively combats UVB-induced skin damage and melanin production, indicating potential for topical skin treatments.
Area Of Science
- Biochemistry
- Dermatology
- Material Science
Background
- Bletilla striata polysaccharides (BSP) possess antioxidant properties but suffer from poor bioaccessibility due to high molecular weight and structure.
- UVB radiation induces skin photoaging and oxidative stress, necessitating effective protective agents.
Purpose Of The Study
- To investigate the anti-photoaging and anti-melanogenesis effects of degraded BSP (DBSPs) on UVB-induced oxidative stress in skin cells.
- To evaluate the structure-activity relationship of DBSPs with varying molecular weights.
Main Methods
- Alkali-assisted extraction of BSP followed by controlled degradation to obtain DBSPs with reduced molecular weights (153.94, 66.96, and 15.54 kDa).
- In vitro assessment of antioxidant activity, cytotoxicity (HSF cells), anti-proliferative effects (B16F10 cells), and protection against UVB-induced oxidative stress.
- Measurement of reactive oxygen species (ROS), melanin content, tyrosinase activity, and malondialdehyde (MDA) levels.
Main Results
- Degradation reduced BSP molecular weight while preserving core structural features, enhancing in vitro antioxidant activity.
- DBSPs exhibited no cytotoxicity to human skin fibroblast (HSF) cells but inhibited melanoma (B16F10) cell proliferation.
- DBSPs protected HSF and B16F10 cells from UVB-induced oxidative stress, reducing ROS and melanin production. DBSP-5 was particularly effective in reducing MDA and inhibiting melanogenesis.
Conclusions
- Degraded Bletilla striata polysaccharides (DBSPs) demonstrate enhanced antioxidant and protective effects against UVB-induced skin damage.
- DBSP-5, with a molecular weight of 15.54 kDa, shows significant potential as a topical therapeutic agent for treating skin conditions related to oxidative stress and photoaging.
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