Related Experiment Video
Updated: May 6, 2026
08:50
5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
Published on: July 3, 2013
23.6K
Targeting Lactobacillus johnsonii to reverse chronic kidney disease
Hua Miao1, Fei Liu2,3,4, Yan-Ni Wang1
1School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
Signal Transduction and Targeted Therapy
|August 4, 2024
Summary
Gut microbial dysbiosis links to chronic kidney disease (CKD) progression. Supplementing Lactobacillus johnsonii and indole-3-aldehyde shows potential for CKD treatment by modulating the aryl hydrocarbon receptor pathway.
Area of Science:
- Microbiology
- Nephrology
- Metabolomics
Background:
- Gut microbial dysbiosis is implicated in chronic kidney disease (CKD) progression.
- Current therapies for progressive CKD remain limited.
- Understanding microbial metabolites' role in kidney disease is crucial.
Purpose of the Study:
- To identify specific gut microbes associated with CKD progression.
- To investigate the renoprotective effects of Lactobacillus johnsonii and indole-3-aldehyde.
- To elucidate the underlying mechanisms involving the aryl hydrocarbon receptor (AHR) pathway.
Main Methods:
- Microbiomic analysis of 480 participants (healthy controls and CKD stages 1-5).
- In vivo studies using adenine-induced CKD, unilateral ureteral obstruction (UUO), and 5/6 nephrectomy (NX) rat models.
- In vitro studies using cultured HK-2 cells.
- Supplementation with L. johnsonii and indole-3-aldehyde (IAld).
- Assessment of kidney lesions, clinical markers, and AHR signaling.
Main Results:
- Lactobacillus johnsonii abundance correlated with CKD progression and kidney markers.
- L. johnsonii supplementation and IAld treatment ameliorated kidney lesions in CKD models.
- IAld suppressed AHR signaling, demonstrating a renoprotective effect.
- L. johnsonii attenuated kidney injury by increasing serum IAld and suppressing AHR signaling.
Conclusions:
- Targeting L. johnsonii offers a potential therapeutic strategy for reversing CKD.
- Microbial tryptophan metabolism, via IAld and AHR signaling, plays a significant role in kidney disease.
- This study identifies novel pathways for CKD prophylaxis and treatment.
Related Concept Videos
01:29Renal Regulation of Acid-Base Balance
2.5K
Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
2.5K
01:30Acute Kidney Injury IV: Diagnostic Studies and Prevention
582
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
582
01:25Chronic Kidney Disease I: Introduction
1.4K
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
1.4K
01:24Chronic Kidney Disease II: Clinical Manifestations
1.1K
Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
1.1K
01:28Chronic Kidney Disease III: Interprofessional Care
706
Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
706
01:18Chronic Kidney Disease IV: Nursing Management
702
Nursing management is essential for preventing complications, maintaining stability, and improving patients' quality of life in chronic kidney disease (CKD). By using a structured approach, nurses help slow CKD progression and support effective patient care.1. Comprehensive patient assessmentEffective management begins with nurses reviewing the patient’s medical history, and identifying key risk factors like diabetes, hypertension, and nephrotoxic drug use. Nurses assess signs of...
702