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Updated: Jun 18, 2025
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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
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Bacillus coagulans-associated severe necrotizing scleral infection
Beau Sylvester1, Spencer D Fuller2, Laura Certain3
1Department of Ophthalmology and Visual Sciences, Washington University School of Medicine in St. Louis, St. Louis, USA.
American Journal of Ophthalmology Case Reports
|August 5, 2024
Summary
Severe scleritis, an eye inflammation, may be caused by Bacillus coagulans bacteria. Metagenomic RNA deep sequencing identified the organism, and treatment with trimethoprim-sulfamethoxazole resolved the infection.
Area of Science:
- Ophthalmology
- Microbiology
- Genomics
Background:
- Scleritis is a severe inflammatory disease of the eye's white outer layer.
- Identifying the causative agent in infectious scleritis can be challenging with conventional methods.
Observation:
- A case of severe scleritis was investigated.
- Standard laboratory tests were inconclusive in identifying the pathogen.
- Metagenomic RNA deep sequencing (MDS) was employed for pathogen discovery.
Findings:
- The infectious agent was identified as Bacillus coagulans using MDS.
- The patient's scleritis resolved following treatment with trimethoprim-sulfamethoxazole.
Implications:
- This case highlights the effectiveness of unbiased, high-throughput sequencing techniques in diagnosing infectious scleritis.
- Metagenomic sequencing offers a powerful tool for identifying novel or difficult-to-culture pathogens in ocular infections.
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