Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GelMA Hydrogels Integrated With aptamer CH6-Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration.

Macromolecular bioscience·2025
Same author

Energy metabolism as therapeutic target for aged wound repair by engineered extracellular vesicle.

Science advances·2024
Same author

Abnormal tooth maturation associated with port wine stains.

Orthodontics & craniofacial research·2023
Same author

EVALUATION OF PRE-ABLATION NLR AND LMR AS PREDICTORS OF DISTANT METASTASES IN PATIENTS WITH DIFFERENTIATED THYROID CANCER.

Acta endocrinologica (Bucharest, Romania : 2005)·2023
Same author

<i>PRRX1</i><sup>+</sup>MSCs Enhance Mandibular Regeneration during Distraction Osteogenesis.

Journal of dental research·2023
Same author

ScRNA-Seq Reveals a Distinct Osteogenic Progenitor of Alveolar Bone.

Journal of dental research·2023

Related Experiment Video

Updated: Jun 18, 2025

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
08:36

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

10.9K

Six1 Regulates Mouse Incisor Development by Promoting Dlx1/2/5 Expression.

S Y Luo1,2,3, S Wang1,2,3, Z X Liu1,2,3

  • 1Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Journal of Dental Research
|August 5, 2024
PubMed
Summary

Transcription factor SIX1 is essential for lower incisor development. SIX1 deletion disrupts dental mesenchyme development by blocking cell fate transitions, impacting tooth formation.

Keywords:
dental mesenchymelower incisor developmentsingle-cell RNA sequencingtoothtranscription factor

More Related Videos

Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment
07:37

Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment

Published on: October 27, 2023

1.4K
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

1.9K

Related Experiment Videos

Last Updated: Jun 18, 2025

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
08:36

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

10.9K
Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment
07:37

Author Spotlight: Understanding Dynamic Cellular Behaviors in Adult Mouse Dental Tissue Renewal and Repairment

Published on: October 27, 2023

1.4K
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

1.9K

Area of Science:

  • Developmental Biology
  • Genetics
  • Oral Biology

Background:

  • Tooth development relies on complex gene regulatory networks involving epithelium and ectomesenchyme.
  • The transcription factor SIX1 is implicated in lower incisor development, but its precise role and mechanisms are unclear.

Purpose of the Study:

  • To elucidate the regulatory role of SIX1 in dental mesenchyme development during incisor formation.
  • To understand the molecular mechanisms by which SIX1 controls cell fate transitions in tooth development.

Main Methods:

  • Utilized Six1 deletion mouse models to study incisor development.
  • Performed single-cell RNA sequencing to create a transcriptome atlas of tooth germ development (bud to bell stage).

Main Results:

  • Identified key transcription factors, including Dlx1/2/5, promoting dental papilla formation from dental ectomesenchyme.
  • Demonstrated that SIX1 deletion impairs the transition from dental ectomesenchyme to dental papilla.
  • Showed SIX1 directly binds to Dlx1/2/5 promoters, driving their co-expression and subsequent epigenetic/transcriptional changes.

Conclusions:

  • SIX1 is indispensable for lower incisor development.
  • SIX1 regulates dental mesenchyme cell fate transitions through the Dlx1/2/5 regulatory network.
  • Findings provide insights into transcription factor-driven networks governing tooth development.