Efficient Light-Based Bioprinting via Rutin Nanoparticle Photoinhibitor for Advanced Biomedical Applications
Feiyi Li1, Xinyue Li1, Shuxin Dai1
1State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300350, China.
ACS Nano
|August 5, 2024
Summary
Rutin nanoparticles (Rnps) improve digital light processing bioprinting by reducing light scattering, enhancing resolution, speed, and cell viability for advanced tissue engineering. This novel photoinhibition strategy offers greater precision and adaptability in creating complex biomimetic constructs.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Digital light processing (DLP) bioprinting offers high resolution and speed for tissue engineering.
- Light scattering in DLP bioprinting limits structural fidelity and printing accuracy.
- Existing photoabsorbers like tartrazine (Tar) have limitations in improving print quality and cytocompatibility.
Purpose of the Study:
- To introduce Rutin nanoparticles (Rnps) as a novel photoinhibition strategy to mitigate light scattering in DLP bioprinting.
- To evaluate the efficacy of Rnps in enhancing printing speed, resolution, homogeneity, and tolerance compared to tartrazine.
- To assess the cytocompatibility and antioxidative properties of Rnps in bioprinted constructs using human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs).
Main Methods:
- Development of Rutin nanoparticles (Rnps) for bioink formulation.
- Incorporation of Rnps into bioink for DLP bioprinting experiments.
- Comparative analysis of printing performance (speed, resolution, homogeneity, tolerance) with Rnps versus tartrazine.
- Assessment of cell viability and resistance to oxidative stress in hiPSC-EC-laden constructs.
Main Results:
- Rnps-infused bioink demonstrated a 1.9× increase in printing speed and a 38.3% improvement in resolution.
- Interlayer homogeneity was enhanced with 58% less overexposure, and print tolerance increased by 3×.
- Rnps significantly improved cytocompatibility and protected hiPSC-ECs from oxidative stress-induced damage.
- Facilitated fabrication of complex, multimaterial, and cell-laden biomimetic constructs.
Conclusions:
- Rutin nanoparticles effectively attenuate light scattering in DLP bioprinting, leading to significant improvements in print fidelity and speed.
- The biocompatible and antioxidative nature of Rnps enhances cell viability and construct stability, crucial for regenerative medicine applications.
- This adaptable photoinhibition strategy holds promise for fabricating intricate biomimetic structures with broad biomedical applications.
