Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of Alumina Nanoparticles Using Plasma-Induced Microbubbles.

Micromachines·2026
Same author

Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis.

Nature communications·2025
Same author

Cytotransducers for Visualization of Spatiotemporal Intercellular Communication.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Development of Repetitive Mechanical Oscillation Needle-Free Injection through Electrically Induced Microbubbles.

Cyborg and bionic systems (Washington, D.C.)·2025
Same author

Cybernetic avatars: Teleoperation technologies from in-body monitoring to social interaction.

Science robotics·2024
Same author

Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation.

Biotechnology letters·2023

Related Experiment Video

Updated: Jun 17, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.1K

Spatiotemporally controlled microvortices provide advanced microfluidic components.

Makoto Saito1, Fumihito Arai2, Yoko Yamanishi1

  • 1Department of Mechanical Engineering, Faculty of Engineering, Kyushu University, Fukuoka 819-0395, Japan.

Proceedings of the National Academy of Sciences of the United States of America
|August 5, 2024
PubMed
Summary

Researchers controlled microvortex development in microchannels using high-speed flows. This created diode-like flow resistance, enabling bidirectional control and single-cell isolation without valves.

Keywords:
flow controlmicrofluidicssingle-cell isolationvortex

More Related Videos

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.3K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K

Related Experiment Videos

Last Updated: Jun 17, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.1K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.3K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K

Area of Science:

  • Fluid dynamics
  • Microfluidics
  • Nonlinear dynamics

Background:

  • Microvortices offer functionality in microchannels via inertia effects like high shear stress and diffusion.
  • Dynamic vortex generation enhances microfluidic applications such as cell stimulation and fluid mixing.
  • Previous research has not explored vortex development at Reynolds numbers in the hundreds within sub-millisecond timescales.

Purpose of the Study:

  • To investigate the modulation of high-speed flows and their impact on microvortex development.
  • To explore the control of spatiotemporal vortex dynamics in microchannels with asymmetric geometries.
  • To demonstrate novel microfluidic functionalities, including diode-like behavior and cell manipulation.

Main Methods:

  • Utilized a piezo-driven on-chip membrane pump to modulate high-speed flows (Reynolds number 54–456) within sub-millisecond timescales.
  • Applied these flows to microchannels with asymmetric geometries to control vortex development.
  • Analyzed the induced pressure losses and direction-dependent flow resistance.

Main Results:

  • Successfully controlled the spatiotemporal development of microvortices in response to oscillatory flow directions.
  • Induced direction-dependent flow resistance, mimicking diode-like behavior.
  • Achieved rectification of oscillatory flow and demonstrated bidirectional flow control without mechanical valves.
  • Showcased application in microfluidic cell pipetting for single-cell isolation.

Conclusions:

  • Modulating high-speed flows enables precise control over microvortex development in microstructures.
  • This approach introduces innovative microfluidic functionalities, including flow rectification and cell isolation.
  • The developed component offers valve-less bidirectional flow control, advancing microfluidic device capabilities.