Related Experiment Video
Updated: Jun 17, 2025
03:48
Restraint to Induce Stress in Mice and Rats
Published on: December 6, 2024
1.2K
Dip2a regulates stress susceptibility in the basolateral amygdala
Jing Li1, Zixuan He, Weitai Chai
1Key Laboratory of Molecular Epigenetics, Ministry of Education; Institute of Genetics and Cytology, Northeast Normal University, Changchun, Jilin Province, China.
Neural Regeneration Research
|August 6, 2024
Summary
Disco-interacting protein 2 homolog A (Dip2a) deficiency in mice alters neurotransmitter metabolism, leading to mood disorder-like behaviors. Dip2a in the basolateral amygdala regulates stress susceptibility.
Area of Science:
- Neuroscience
- Metabolomics
- Molecular Biology
Background:
- Neurotransmitter metabolism dysregulation is linked to mood disorders like depression and anxiety.
- Disco-interacting protein 2 homolog A (Dip2a) knockout mice show brain development and amino acid metabolism abnormalities.
- Dip2a's role in neurotransmitter metabolism, particularly amino acids, is suggested.
Purpose of the Study:
- To investigate the role of Dip2a in neurotransmitter metabolism and its connection to affective disorders.
- To analyze the impact of Dip2a deficiency on tryptophan and thyroxine metabolism in specific brain regions.
- To explore the function of Dip2a in the basolateral amygdala concerning stress and mood regulation.
Main Methods:
- Targeted neurotransmitter metabolomics analysis in Dip2a knockout mice.
- Assessment of neurotransmitter levels (e.g., 5-hydroxytryptamine) following acute restraint stress.
- Behavioral testing (tail suspension test) in mice with Dip2a deletion in specific neuronal populations.
Main Results:
- Dip2a deficiency led to altered tryptophan and thyroxine metabolism in the basolateral amygdala and medial prefrontal cortex.
- Acute stress decreased 5-hydroxytryptamine levels in the basolateral amygdala.
- Deletion of Dip2a in basolateral amygdala excitatory neurons induced hopelessness-like behavior.
Conclusions:
- Dip2a plays a role in regulating amino acid and thyroxine metabolism, impacting affective disorder pathways.
- Dip2a in the basolateral amygdala excitatory neurons is crucial for modulating stress susceptibility.
- These findings highlight Dip2a as a potential factor in the pathophysiology of affective disorders.
Related Concept Videos
01:37Hypothalamic-Pituitary Axis
59.8K
The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
59.8K
01:24Physiological Foundation of Stress
54
Stress triggers a coordinated physiological response involving the sympathetic nervous system (SNS) and the hypothalamic-pituitary-adrenal (HPA) axis. This dual activation ensures that the body is prepared for both immediate and prolonged stress management. The process begins with the perception of a stressor. This initial phase activates the SNS, leading to the rapid release of adrenaline (epinephrine) from the adrenal glands.
Role of the Sympathetic Nervous System
Adrenaline triggers the...
Role of the Sympathetic Nervous System
Adrenaline triggers the...
54
01:16Role of Amygdala in Memory
189
The amygdala is a small, almond-shaped structure responsible for processing and storing memories, particularly those linked to emotions like fear and stress. It plays an essential role in the brain's response to emotionally significant events and often enhances memory formation by triggering stress hormone release. The amygdala is vital for encoding and retrieving memories associated with fear or stress, a process that is adaptive by helping organisms avoid dangerous situations.
One of the...
One of the...
189