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Updated: Jun 17, 2025

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

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Published on: February 17, 2023

Induced Periodontitis in Rats With Three Ligature Types: An Exploratory Study.

Natalia Chatzaki¹, Andreas Stavropoulos^2,3,4,5, Balazs Denes⁶

¹Division of Regenerative Dental Medicine and Periodontology, University Clinics of Dental Medicine, University of Geneva, Geneva, Switzerland.

Clinical and Experimental Dental Research

|August 6, 2024

Summary

Metal wires and silk ligatures showed minimal differences in inducing periodontal bone loss in rats. Further studies are needed to confirm findings in this experimental periodontitis model.

Keywords:

experimental periodontitis ligatures metal wire rats

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Area of Science:

Periodontology
Oral Biology
Biomaterials Science

Background:

Cervical ligature placement in rat molars is a common model for inducing experimental periodontitis.
Understanding ligature efficacy is crucial for reliable periodontitis research.

Purpose of the Study:

To compare the effectiveness of metal wires (MWs) and silk ligatures (SLs) in inducing periodontal bone loss in rats.
To evaluate the impact of sandblasting on metal wire efficacy.

Main Methods:

Wistar rats were divided into three groups receiving MWs, sandblasted MWs (SMW), or SLs around a mandibular molar.
Control teeth were left without ligatures.
Periodontal bone loss was measured using micro-computed tomography (micro-CT) scans at 24 and 35 days post-placement.

Main Results:

Ligature retention varied, with MWs showing the highest retention, followed by SMW, and then SLs.
Minimal bone loss was observed across all groups, with no significant differences between ligated and control teeth.
In most cases, the difference in crestal bone level was less than 0.20 mm.

Conclusions:

None of the tested ligatures, including MWs and SLs, effectively induced significant periodontal bone loss in this rat model.
Metal wires, even without sandblasting, do not appear to be a superior alternative to silk ligatures for this experimental periodontitis model.
Larger, well-powered studies are recommended to validate these findings.