Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

D-Amino Acids in Marine and Terrestrial Environments: Diversity and Uniformity of Their Microbial Catabolism.

Environmental microbiology·2026
Same author

Multiple novel membrane proteins involve phthalate ester degradation in <i>Rhodococcus</i> sp. AH-ZY2.

Applied and environmental microbiology·2026
Same author

Morphological plasticity of endophytic Chitinophaga pinensis.

Antonie van Leeuwenhoek·2026
Same author

Development and Application of SRCA for Rapid Detection of Pseudomonas aeruginosa in Bottled Drinking Water.

Molecular biotechnology·2026
Same author

Controlled Rigidity Nanolipogel-Mediated Topical Delivery of Fucosterol for Treating Androgenic Alopecia through Follicle Targeting, Promoting Angiogenesis and Inhibiting Inflammation.

Biomacromolecules·2026
Same author

Cold-brewed yellow tea showed stronger inhibition on high-fat diet induced mice obesity than that hot brewed, due to higher non-ester catechins content based on metabolomic profile.

3 Biotech·2025

Related Experiment Video

Updated: Jun 17, 2025

Author Spotlight: Development of Homogeneous &#954;-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting
04:58

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting

Published on: May 3, 2024

1.1K

Cell-Based Meat Scaffold Based on a 3D-Printed Starch-Based Gel.

Jing Wang1, Siqing Dai1, Ning Xiang2

  • 1Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China.

Journal of Agricultural and Food Chemistry
|August 6, 2024
PubMed
Summary

Researchers developed a novel starch-based gel scaffold using 3D printing for cell-based meat production. This scaffold supports C2C12 myoblast proliferation and differentiation, showing promise for sustainable meat alternatives.

Keywords:
3D printingcell-based meatgelscaffoldsstarch

More Related Videos

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
06:36

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Published on: April 24, 2019

9.6K
Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
07:26

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

Published on: May 26, 2023

2.4K

Related Experiment Videos

Last Updated: Jun 17, 2025

Author Spotlight: Development of Homogeneous &#954;-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting
04:58

Author Spotlight: Development of Homogeneous κ-Carrageenan Sub-Microgel Baths for High-Resolution 3D Bioprinting

Published on: May 3, 2024

1.1K
3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
06:36

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Published on: April 24, 2019

9.6K
Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
07:26

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

Published on: May 26, 2023

2.4K

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Food Science

Background:

  • Developing sustainable and scalable methods for cell-based meat production is crucial.
  • Biocompatible scaffolds are essential for providing structural support and promoting cell growth in cultured meat.
  • Starch-based materials offer potential as biodegradable and customizable scaffold components.

Purpose of the Study:

  • To develop and optimize a 3D-printed starch-based gel scaffold for cell-based meat cultivation.
  • To investigate the effects of calcium carbonate nanoparticles-glucono delta lactone (CaCO3 NPs-GDL) on scaffold properties.
  • To evaluate the scaffold's suitability for supporting C2C12 mouse skeletal myoblast proliferation and differentiation.

Main Methods:

  • Fabrication of starch-based gel ink and 3D printing of scaffolds with optimized infill density.
  • Incorporation of CaCO3 NPs-GDL to modify scaffold characteristics.
  • Assessment of scaffold properties including swelling, in vitro digestion, water stability, pore size, and compression modulus.
  • Cultivation of C2C12 cells on scaffolds and evaluation of cell proliferation, fusion, and maturation.

Main Results:

  • Optimized 3D-printed scaffolds (50% infill density) exhibited a highly ordered microstructure.
  • Addition of 0.075 g CaCO3 NPs enhanced scaffold properties, including pore size (80-120 μm) and compression modulus (246.76 Pa).
  • C2C12 cells showed a 2.81-fold increase in number, with fusion and maturation indices of 57.00% and 34.56%, respectively, indicating successful proliferation and differentiation.

Conclusions:

  • The developed starch-based composite gel scaffold is suitable for cell-based meat production.
  • The scaffold demonstrates excellent water stability, in vitro degradability, and supports C2C12 cell growth and differentiation.
  • This research presents a promising approach for the sustainable manufacturing of cell-based meat products.