Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell-Free Protein Synthesis in Porous Parylene Scaffolds.

Small methods·2026
Same author

Surface-Capped Protein Nanoparticles for Nonviral Gene Delivery.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A Tandem Chemical Vapor Deposition Platform for the Solvent-Free Synthesis of Polypeptide Architectures.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Engineered Fenretinide- and Tocilizumab-Releasing Janus Nanoparticles for Site-Directed Immunochemoprevention of Squamous Cell Carcinoma of the Lung.

Pharmaceutics·2025
Same author

Autophagy Upregulation in Mutant Isocitrate Dehydrogenase 1 (IDH1) Glioma Uncovers a Novel Therapeutic Target.

Research square·2025
Same author

Circularly Polarized Light Emission From Single Chiral Hedgehog Particles Coated with Nanofilms of Achiral Perovskites.

Advanced materials (Deerfield Beach, Fla.)·2025
Same journal

Electrospun Liquid Crystal Elastomers as Stress-Free Thermo- and Photoresponsive Actuators.

ACS applied materials & interfaces·2026
Same journal

Tunable Electrical Transport and Magnetic Anisotropy in Textured SrRuO<sub>3</sub> Films Mediated by Gap Control of Monolayer Ca<sub>2</sub>Nb<sub>3</sub>O<sub>10</sub> Nanosheet Templates.

ACS applied materials & interfaces·2026
Same journal

Label-Free Capacitive Immunosensing of Lactate Dehydrogenase and Interleukin-6 Using a Protein-Passivated Graphene Interface.

ACS applied materials & interfaces·2026
Same journal

Improved Carrier Transport and Enhanced Detection Sensitivity Through Zr<sup>4+</sup> Doping in LiYMo<sub>2</sub>O<sub>8</sub> Single Crystals for X-ray Detectors.

ACS applied materials & interfaces·2026
Same journal

Near-Infrared Light-Driven Microgrooved UCNPs/Azobenzene-LCE Actuators and Substrates for Cardiomyoblast Alignment.

ACS applied materials & interfaces·2026
Same journal

Recent Advances in Superlattice-Based Thermoelectrics.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2025

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

13.9K

Self-Reporting Therapeutic Protein Nanoparticles.

Anthony J Berardi1,2, Jeffery E Raymond2,3,4, Albert Chang2,5

  • 1Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, Michigan 48105, United States.

ACS Applied Materials & Interfaces
|August 6, 2024
PubMed
Summary
This summary is machine-generated.

We developed fluorescent nanoparticle sensors that track drug release by monitoring changes in their environment. Protein nanoparticles showed higher sensitivity, enabling better understanding of nanoparticle-drug interactions for improved drug delivery systems.

Keywords:
drug deliveryfluorescence lifetimenanoparticleself-reportingtheranostics

More Related Videos

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

28.2K
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

15.9K

Related Experiment Videos

Last Updated: Jun 17, 2025

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

13.9K
Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

28.2K
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

15.9K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Nanoparticle sensors offer potential for monitoring complex chemical environments.
  • Fluorescence lifetime analysis provides a sensitive method for probing molecular interactions.
  • Understanding nanoparticle-drug interactions is crucial for developing effective drug delivery systems.

Purpose of the Study:

  • To develop modular nanoparticle sensors with fluorescent reporters for analyzing interparticle chemical environments.
  • To compare the sensitivity of different nanoparticle types (protein, polymer nanogels, block copolymer micelles) in detecting environmental changes.
  • To investigate the utility of these sensors in monitoring drug encapsulation and release profiles.

Main Methods:

  • Synthesis of nanoparticle sensors functionalized with dithiomaleimide-based fluorescent molecular reporters.
  • Utilizing time-resolved fluorescence spectroscopy for fluorescence lifetime analysis.
  • Encapsulation of paclitaxel (PTX) in self-reporting protein nanoparticles to study drug-induced changes.

Main Results:

  • Protein nanoparticles demonstrated superior sensitivity to core environmental changes compared to polymer nanogels and block copolymer micelles.
  • Paclitaxel encapsulation in protein nanoparticles induced distinct fluorescence lifetime signatures.
  • Differences in fluorescence lifetime profiles correlated with known burst- vs. diffusion-controlled drug release mechanisms.

Conclusions:

  • Self-reporting protein nanoparticles can effectively monitor drug encapsulation and release kinetics.
  • These sensors are valuable tools for studying nanoparticle stability and nanoparticle-drug interactions.
  • The developed strategy facilitates the rational design of advanced nanoparticle-based drug carriers.