Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
RNA Splicing01:32

RNA Splicing

56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.0K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

879
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
879
pre-mRNA Processing02:01

pre-mRNA Processing

52.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic Analyses and Weighted Gene Co-Expression Network Analysis (WGCNA) Identify Key Drought-Responsive Genes in Rice Roots (<i>Oryza sativa</i> L.) Under PEG Treatment.

Plants (Basel, Switzerland)·2026
Same author

Metabolic Fingerprinting of Urine Reveals Metabolite Changes in Women With Breast Cancer.

Cancer medicine·2026
Same author

Differential Jasmonate Profiles in Oat Roots and Leaves Reveal a Role for 12-Oxo Phytodienoic Acid (OPDA) in Drought Tolerance by Modulating Root Growth.

Plants (Basel, Switzerland)·2026
Same author

The Urinary Proteome Differs with the Presence and Type of Breast Cancer.

Journal of proteome research·2025
Same author

Plant-pathogen interactions: making the case for multi-omics analysis of complex pathosystems.

Stress biology·2025
Same author

Phytoglobin Scavenging of Nitric Oxide Is Associated With Ethylene Reduction and Drought Tolerance in Oat (Avena sativa).

Physiologia plantarum·2025

Related Experiment Video

Updated: Jun 17, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

6.5K

The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare).

Ewa Sybilska1, Anna Collin1, Bahareh Sadat Haddadi2

  • 1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.

Scientific Reports
|August 6, 2024
PubMed
Summary

The cap-binding complex (CBC) regulates seed germination by interacting with abscisic acid (ABA). Double mutants show ABA insensitivity, unlike single mutants, revealing CBC

Keywords:
ABAAlternative splicingBarleyCap-binding complexEmbryoGerminationTranscriptome

More Related Videos

A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
08:52

A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana

Published on: November 9, 2013

13.5K
Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

2.2K

Related Experiment Videos

Last Updated: Jun 17, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

6.5K
A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
08:52

A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana

Published on: November 9, 2013

13.5K
Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

2.2K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Seed germination is a crucial developmental process.
  • Abscisic acid (ABA) is a key plant hormone regulating seed dormancy and germination.
  • The cap-binding complex (CBC), composed of CBP20 and CBP80, is involved in RNA processing.

Purpose of the Study:

  • To investigate the role of the cap-binding complex (CBC) in modulating abscisic acid (ABA) effects on barley seed germination.
  • To elucidate the molecular mechanisms underlying CBC's function in ABA signaling.

Main Methods:

  • Utilized single and double barley mutants in genes encoding CBC components (CBP20 and CBP80).
  • Performed transcriptome and metabolome analysis on mutant lines.
  • Assessed germination phenotypes under varying ABA concentrations.

Main Results:

  • The double mutant hvcbp20.ab/hvcbp80.b exhibited ABA insensitivity during germination.
  • Single mutants displayed ABA hypersensitivity, contrasting with the double mutant phenotype.
  • Transcriptome and metabolome analyses revealed significant changes in gene expression, splicing, and identified crosstalk between CBC, ABA, and brassinosteroid (BR) signaling.

Conclusions:

  • The CBC plays a critical and complex role in regulating ABA sensitivity during barley seed germination.
  • CBC's function is essential for proper ABA signaling, with distinct roles for CBP20 and CBP80.
  • The study highlights an intricate regulatory network involving CBC, ABA, and BR pathways impacting plant development.