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Engineering a dysbiotic biofilm model for testing root caries interventions through microbial modulation.

Naile Dame-Teixeira1,2, Reem El-Gendy3,4, Andressa Souza de Oliveira5

  • 1Department of Dentistry, School of Health Sciences, University of Brasilia, Brasilia, Brazil. nailedame@unb.br.

Microbiome
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PubMed
Summary
This summary is machine-generated.

This study optimized an in vitro root caries model to test microbial modulation strategies. Pre-treating with natural compounds before biofilm challenges proved more effective than post-treatment for reversing dysbiosis.

Keywords:
BiofilmDental cariesDysbiosisIn vitro modelOral microbiomePlant extractsRoot caries

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Area of Science:

  • Oral microbiology
  • Biomaterials science
  • Translational research

Background:

  • Developed a dysbiotic biofilm model for in vitro root caries.
  • Utilized saliva inoculum from volunteers to create complex biofilms.
  • Mimicked cariogenic conditions using sucrose cycles.

Purpose of the Study:

  • To engineer and optimize an in vitro root caries model.
  • To investigate microbial modulation strategies for root caries.
  • To compare pre-treatment and post-treatment efficacy of natural compounds.

Main Methods:

  • Grew complex biofilms on bovine root slabs or coated devices.
  • Applied natural compounds using pre-treatment and post-treatment strategies.
  • Analyzed metatranscriptome, enzyme activity, SEM, and micro-computed tomography (μ-CT).

Main Results:

  • Achieved complex, diverse biofilms with bacteria, archaea, and viruses.
  • Observed differential gene expression of carbohydrate esterases and glucosyltransferases.
  • Pre-treatment with cranberry reduced microbial viability and gelatinase activity.

Conclusions:

  • The optimized root caries model can test in vitro microbial modulation.
  • Pre-treatments were more effective than post-treatments for dysbiosis reversal.
  • Findings support developing varnish products for tooth prophylaxis.