Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

2.5K
2.5K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracellular delivery of full-length antibodies via organ-targeted lipid nanoparticles.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

AI-Designed Peptides as Tools for Biochemistry.

Biochemistry·2026
Same author

Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst.

bioRxiv : the preprint server for biology·2026
Same author

Development of nanobody-conjugated LL37 for synergistic therapy against MDR <i>Acinetobacter baumannii</i>.

mSphere·2026
Same author

Isolation of Antigen-Specific Nanobodies From Synthetic Libraries Using a Protein Selection Strategy That Combines MACS-Based Screening of YSD and FLI-TRAP.

Bio-protocol·2026
Same author

A foundation model for microbial growth dynamics.

bioRxiv : the preprint server for biology·2026
Same journal

HIV Transmission Dynamics in Greater Mexico City are Shaped by Dense Spatial Mixing.

Research square·2026
Same journal

A UCP1-IRES-Cre Knock-In Mouse Enables Specific Brown Adipocyte Targeting Without CNS Off-Target Expression.

Research square·2026
Same journal

Precision RNAi for Fibrodysplasia Ossificans Progressiva: a combinatorial, unimolecular, allele selective approach.

Research square·2026
Same journal

Perceptions of end-of-life care quality among bereaved closest contacts of community-dwelling older Australians: a cross-sectional survey of the ASPREE cohort.

Research square·2026
Same journal

Heavy-chain immune repertoire sequencing enables language-model prediction of antigen-specific antibodies.

Research square·2026
Same journal

25+ Years of TRPV4: From Discovery to Translational Horizons.

Research square·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K

Programmable Protein Stabilization with Language Model-Derived Peptide Guides.

Lauren Hong1, Tianzheng Ye2, Tian Zi Wang1

  • 1Department of Biomedical Engineering, Duke University.

Research Square
|August 7, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed deubiquibodies (duAbs) for targeted protein stabilization (TPS). This new method uses engineered proteins to stabilize disease-related proteins, offering a programmable approach for difficult-to-treat conditions.

More Related Videos

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

2.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.7K

Related Experiment Videos

Last Updated: Jun 17, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K
Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

2.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Dysregulated protein degradation is implicated in diseases like cancer and neurodegeneration.
  • Targeted protein stabilization (TPS) is a therapeutic strategy, but current methods have limitations.
  • Existing TPS modalities use limited chemical linkers and warheads, hindering application to new or
  • undruggable
  • targets.

Purpose of the Study:

  • To engineer a novel, modular system for targeted protein stabilization (TPS).
  • To expand the range of druggable targets for protein stabilization therapies.
  • To develop a programmable, CRISPR-analogous strategy for therapeutic intervention.

Main Methods:

  • Engineered deubiquibodies (duAbs) by fusing computationally designed peptides to the OTUB1 deubiquitinase catalytic domain.
  • Validated duAb efficacy in stabilizing exogenous and endogenous proteins in human cells.
  • Demonstrated modularity by designing new target-binding peptides using generative language models.

Main Results:

  • Deubiquibodies (duAbs) effectively stabilized target proteins in a deubiquitinase-dependent manner.
  • The duAb system demonstrated modularity, enabling stabilization of diverse targets including p53, WEE1, and PAX3::FOXO1.
  • Successfully stabilized key tumor suppressors and disordered oncoproteins, expanding TPS applicability.

Conclusions:

  • The engineered deubiquibody (duAb) system provides a versatile and programmable platform for targeted protein stabilization (TPS).
  • This approach overcomes limitations of small-molecule-based TPS, offering a new strategy for diseases driven by protein dysregulation.
  • Deubiquibodies represent a significant advancement in developing novel therapeutics for challenging diseases.