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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Jun 17, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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Advancing PAM-less genome editing in soybean using CRISPR-SpRY.

Xiao Chen1, Zhaohui Zhong2, Xu Tang2,3

  • 1Key Laboratory of Soybean Molecular Design Breeding, National Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.

Horticulture Research
|August 7, 2024
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Summary

Scientists developed a novel CRISPR-Cas9 system using SpRY in soybean, overcoming protospacer adjacent motif limitations for efficient genome editing. This PAM-less tool enhances soybean genetic improvement and breeding precision.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • CRISPR-Cas9 technology is crucial for soybean genetic improvement.
  • Classical CRISPR-Cas9 systems face limitations in soybean's complex genome due to protospacer adjacent motif (PAM) requirements.
  • Targeted editing of specific loci in soybean remains challenging.

Purpose of the Study:

  • To develop and demonstrate a novel, PAM-less genome editing system for soybean using the SpRY protein.
  • To evaluate the efficiency and accuracy of SpRY-mediated targeted mutagenesis and base editing in soybean.
  • To overcome existing PAM barriers in soybean genome editing.

Main Methods:

  • Development of a SpRY-mediated genome editing system for soybean.
  • Targeted mutagenesis of representative agronomic trait genes in soybean.
  • Application of SpRY-based cytosine base editor (SpRY-hA3A) for C-to-T conversion.
  • Application of SpRY-based adenine base editor (SpRY-ABE8e) for A-to-G conversion.

Main Results:

  • SpRY protein achieved efficient targeted mutagenesis at relaxed PAM sites in soybean.
  • SpRY-hA3A accurately induced C-to-T base conversions in soybean.
  • SpRY-ABE8e accurately induced A-to-G base conversions in soybean.
  • The SpRY toolbox enables PAM-free editing of the soybean genome.

Conclusions:

  • The SpRY toolbox offers a versatile and efficient PAM-less genome editing solution for soybean.
  • This technology overcomes restrictive PAM barriers, expanding soybean genome editing capabilities.
  • The SpRY system holds significant practical value for precise soybean breeding and molecular design.