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Related Experiment Video

Updated: Jun 17, 2025

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images
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PidTools: Algorithm and web tools for crop pedigree identification analysis.

Yunlong Zhang1, Yikun Zhao1, Shipeng Ma1

  • 1Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Key Laboratory of Crop DNA Fingerprinting Innovation and Utilization (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shuguang Garden Middle Road No. 9, Beijing 100097, China.

Computational and Structural Biotechnology Journal
|August 7, 2024
PubMed
Summary
This summary is machine-generated.

PidTools accurately identifies crop pedigrees using genomic data, aiding breeders in genetic improvement. This tool reconstructs and visualizes plant lineage, crucial for understanding crop evolution and maximizing heterosis.

Keywords:
Crop pedigreesGermplasm resource evaluationMolecular breedingPedigree identification algorithm

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Area of Science:

  • Plant genetics and breeding
  • Bioinformatics
  • Genomic analysis

Background:

  • Accurate crop pedigrees are essential for genetic improvement and heterosis exploitation.
  • Missing genealogical information hinders effective germplasm selection and breeding strategies.

Purpose of the Study:

  • To develop an algorithm (PidTools) for accurate pedigree identification and reconstruction in crops.
  • To validate the PidTools algorithm using maize genomic data.
  • To provide user-friendly tools for analyzing and visualizing plant breeding material pedigrees.

Main Methods:

  • Development of PidTools, a suite of five algorithms across three core modules.
  • Construction of a genotype database from 5791 maize inbred lines using Maize6H-60K array data.
  • Validation of PidTools for identifying pedigrees of individual lines (Jing72464, Zheng58) and groups (doubled-haploid, Dan340 derived lines).

Main Results:

  • Successfully identified the genome-wide pedigree for maize inbred lines without prior parental information.
  • Determined parents of a doubled-haploid group using genotyping data.
  • Visualized the pedigree of 21 Dan340 derived lines.

Conclusions:

  • PidTools provides accurate pedigree identification and reconstruction for all crops.
  • The developed online platform (PidTools-WS) and toolkit (PidTools-CLI) offer valuable resources for maize breeding.
  • This technology facilitates better understanding of crop kinship and evolutionary history for enhanced breeding programs.