Multiplexed CRISPR-Cas system targeting ASFV genes in vivo: solution lies within

Area of Science:

• Genetics and Genomics
• Veterinary Virology
• Biotechnology

Background:

• African swine fever virus (ASFV) presents a major threat to global swine production.
• Effective viral containment and control strategies are urgently needed.
• CRISPR-Cas gene editing offers potential for developing disease-resistant livestock.

Purpose of the Study:

• To establish and evaluate a multiplexed CRISPR-Cas system for targeting ASFV.
• To assess the antiviral activity of the CRISPR-Cas system in vitro and in vivo.
• To explore germline editing as a strategy for ASFV resistance in pigs.

Main Methods:

• Development of a multiplexed CRISPR-Cas system targeting the ASFV genome.
• In vitro testing of the system's efficacy in reducing viral replication.
• Generation of germline-edited pigs expressing the CRISPR-Cas system for in vivo evaluation.

Main Results:

• Significant reduction in ASFV replication was observed in vitro.
• Germline-edited pigs maintained normal growth and continuous guide RNA expression.
• No survival advantage was detected in ASFV-challenged edited pigs compared to controls.

Conclusions:

• This study represents the first attempt at germline editing for ASFV resistance in pigs.
• The CRISPR-Cas system demonstrated in vitro efficacy against ASFV.
• Further research is needed to enhance ASFV resistance through gene editing in livestock.