Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

737
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
737
Quantitative Analysis01:12

Quantitative Analysis

265
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
265
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
Qualitative Analysis01:10

Qualitative Analysis

232
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
232

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maximizing Lipidome Coverage of Mouse Liver Following the IV Administration of Gefitinib by Combining Both UHPLC-MS-Based Untargeted and Targeted Lipidomics.

Journal of proteome research·2026
Same author

Metabolism and Excretion of Synthetic Extended Viperin Pathway Deoxydidehydronucleosides in the Sprague-Dawley Rat.

Journal of proteome research·2026
Same author

Best Practices in GC-MS and GC × GC-MS-Based Metabolomics and Volatile Analyses: An International Survey.

Analytical chemistry·2026
Same author

Correction: What's in a name? Metabolite identification: challenges and pitfalls in untargeted metabolomics.

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

What's in a name? Metabolite identification: challenges and pitfalls in untargeted metabolomics.

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

Endocrine and metabolic determinants of cardiometabolic risk in mild autonomous cortisol secretion.

EBioMedicine·2026
Same journal

Next-generation newborn screening: feasibility of combined genetic and biochemical testing for 95 treatable inherited metabolic disorders.

Metabolomics : Official journal of the Metabolomic Society·2026
Same journal

A priori sample size determination and power analysis in metabolic phenotyping and integrative metabolomics: an application framework based on a systematic review of literature.

Metabolomics : Official journal of the Metabolomic Society·2026
Same journal

Untargeted metabolomics and proteomics reveals cocoa-mediated mitigation of valproic acid-induced dysregulation in a zebrafish model of autism: pilot study.

Metabolomics : Official journal of the Metabolomic Society·2026
Same journal

Lipidomics of longer-term effects in the murine model after exposure to mixed field radiation and combined infection.

Metabolomics : Official journal of the Metabolomic Society·2026
Same journal

Evaluation of metabolite biomarker candidates in detecting HCC in patients with liver cirrhosis.

Metabolomics : Official journal of the Metabolomic Society·2026
Same journal

Metabolic pathways associated with cardiometabolic risk effects on cognition in middle-aged adults: the CARDIA study.

Metabolomics : Official journal of the Metabolomic Society·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K

Analysis types and quantification methods applied in UHPLC-MS metabolomics research: a tutorial.

Richard D Beger1, Royston Goodacre2, Christina M Jones3

  • 1National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, 72079, USA.

Metabolomics : Official Journal of the Metabolomic Society
|August 7, 2024
PubMed
Summary
This summary is machine-generated.

This tutorial clarifies analytical methods in metabolomics, comparing untargeted, targeted, and semi-targeted approaches. It explains how to determine metabolite concentrations, highlighting accuracy and precision differences.

Keywords:
MetabolomicsQuantificationSemi-targetedTargetedUHPLC-MSUntargetedValidation

More Related Videos

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.4K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.7K

Related Experiment Videos

Last Updated: Jun 17, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.4K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.7K

Area of Science:

  • Metabolomics and Lipidomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Untargeted, targeted, and semi-targeted analytical methods are used in metabolomics and lipidomics.
  • Ultra High Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) is a key technology for metabolite detection.
  • Quantification methods report either normalized peak area or absolute concentration.

Purpose of the Study:

  • Define similarities and differences between analytical approaches in metabolomics.
  • Explain how to determine absolute metabolite concentrations.
  • Discuss advantages and limitations regarding accuracy and precision.

Main Methods:

  • Comparison of untargeted, targeted, and semi-targeted metabolomics strategies.
  • Evaluation of UHPLC-MS for metabolite analysis.
  • Review of quantification techniques for metabolite concentrations.

Main Results:

  • Untargeted methods provide peak responses and fold changes, even for unknown metabolites.
  • Targeted and semi-targeted methods require prior analyte knowledge.
  • Absolute concentration determination necessitates validated, analyte-specific methods for accuracy and precision.

Conclusions:

  • Method selection depends on metabolite knowledge and reporting requirements.
  • Accurate metabolite quantification requires rigorous method validation.
  • Understanding analytical approaches is crucial for reliable metabolomics data.