Related Experiment Video
Updated: Jun 17, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Tailoring the Performance of 19F-Labeled Probes via Dynamic Covalent Chemistry for Improved Chiral Discrimination
Jiajin Weng1, Lixian Wen1, Guangxing Gu1
1Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Abstract:
This work presents a novel strategy for postmodifying probes using dynamic covalent chemistry. Leveraging reversible interactions between boronic acid and diols, we obtained a panel of 19F-labeled probes with distinct resolving abilities. This approach enables rapid identification of probes with satisfactory performance, streamlining synthesis, and enhancing efficiency in chiral analysis. Our findings demonstrate an exceptional ability to differentiate compounds with distal chirality and challenging aliphatic amines. The postmodified probes also exhibit accuracy and reliability in determining enantiomeric excess, promising advancements in enantio-analysis techniques and chiral discrimination.
More Related Videos
14:36Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
14:02Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
Published on: April 9, 2018
Related Concept Videos
Prochirality
Fischer Projections
¹H NMR Chemical Shift Equivalence: Enantiotopic and Diastereotopic Protons
In chiral compounds such as 2-butanol, replacing the methylene hydrogens at C3 produces a pair of...
Chirality in Nature
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...