Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Vitro Selection of Antibodies Targeting <i>Yersinia pestis</i> Membrane Lipids Using Nanodisc-Based Antigen Presentation.

Pathogens (Basel, Switzerland)·2026
Same author

Editorial: Towards the rapid and systematic assessment of vaccine technologies.

Frontiers in immunology·2026
Same author

Ticking off Lyme disease: OspA mRNA vaccine halts infection in mouse model.

Molecular therapy. Nucleic acids·2025
Same author

A consensus mathematical model of vaccine-induced antibody dynamics for multiple vaccine platforms and pathogens.

Frontiers in immunology·2025
Same author

Prediction of Specificity of α-Conotoxins to Subtypes of Human Nicotinic Acetylcholine Receptors with Semi-supervised Machine Learning.

ACS chemical neuroscience·2025
Same author

Evaluating the factors influencing accuracy, interpretability, and reproducibility in the use of machine learning classifiers in biology to enable standardization.

Scientific reports·2025

Related Experiment Video

Updated: Jun 17, 2025

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
08:24

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Published on: November 19, 2020

3.3K

Nanodisc assembly from bacterial total lipid extracts.

Trent R Llewellyn1, Olivia R C Pimentel1, Kiersten D Lenz1

  • 1Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 United States.

Chemistry and Physics of Lipids
|August 7, 2024
PubMed
Summary

Researchers created bacterial lipid nanodiscs from Yersinia pestis. This method enables membrane protein studies and vaccine discovery without using live pathogens.

Keywords:
Amphiphilic antigenLipid biochemistryNanodiscNanolipoproteinSynthetic lipoproteinTotal lipid extractYersinia pestis

More Related Videos

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
12:25

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Published on: August 31, 2012

25.5K
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.2K

Related Experiment Videos

Last Updated: Jun 17, 2025

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
08:24

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Published on: November 19, 2020

3.3K
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
12:25

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Published on: August 31, 2012

25.5K
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.2K

Area of Science:

  • Biochemistry
  • Membrane Biology
  • Nanotechnology

Background:

  • Nanodiscs are discoidal lipoproteins used to study membrane proteins in their native state.
  • Current nanodiscs are typically synthesized using synthetic lipids.
  • Native lipids can also be studied using nanodiscs, offering insights into their natural configuration.

Purpose of the Study:

  • To develop a method for synthesizing nanodiscs from bacterial total lipid extracts.
  • To utilize Yersinia pestis, a biothreat agent, as a proof-of-concept for bacterial nanodisc synthesis.
  • To enable membrane characterization and vaccine antigen discovery using bacterial lipids without live pathogen concerns.

Main Methods:

  • Extraction of total lipids from Yersinia pestis.
  • Self-assembly of bacterial lipids into nanodiscs.
  • Characterization of bacterial lipid nanodiscs.

Main Results:

  • Successful synthesis of nanodiscs composed entirely of bacterial lipids.
  • Demonstration of Yersinia pestis lipids forming stable nanodisc structures.
  • Validation of the approach for potential vaccine antigen discovery.

Conclusions:

  • Bacterial total lipid extracts can be effectively used to synthesize nanodiscs.
  • This method provides a safer alternative for studying bacterial membrane proteins and antigens.
  • Bacterial lipid nanodiscs offer a promising platform for membrane research and vaccine development.