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TRIM27 promotes the Warburg effect and glioblastoma progression via inhibiting the LKB1/AMPK/mTOR axis

Juexian Xiao1, Haitao Luo1, Shikai Gui1

  • 1Department of Neurosurgery, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University Nanchang 330006, Jiangxi, China.

American Journal of Cancer Research
|August 8, 2024
PubMed

Insights

Tripartite motif 27 (TRIM27) promotes glioblastoma growth by enhancing the Warburg effect and inhibiting the LKB1/AMPK/mTOR pathway. TRIM27 may serve as a diagnostic and therapeutic target for glioblastoma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Altered protein ubiquitination is a hallmark of cancer.
  • Tripartite motif (TRIM) E3 ubiquitin ligases are implicated in tumor development, growth, and metastasis.
  • TRIM27 is a potential tumor promoter, but its role in glioblastoma (GBM) is largely unknown.

Purpose of the Study:

  • To investigate the biological functions and clinical relevance of TRIM27 in glioblastoma.
  • To elucidate the molecular mechanisms underlying TRIM27's role in GBM pathogenesis.

Main Methods:

  • Analysis of TRIM27 expression in GBM tissues and cell lines.
  • In vitro and in vivo functional assays to assess the impact of TRIM27 deletion on GBM cell growth.
  • Investigation of TRIM27's effect on the Warburg effect and the LKB1/AMPK/mTOR signaling pathway.
  • Co-immunoprecipitation assays to determine the interaction between TRIM27 and LKB1.

Main Results:

  • TRIM27 expression is elevated in GBM tissues and cell lines.
  • TRIM27 deletion significantly inhibits GBM cell proliferation in vitro and tumor growth in vivo.
  • TRIM27 promotes GBM growth by enhancing the Warburg effect via inactivation of the LKB1/AMPK/mTOR pathway.
  • TRIM27 directly binds to LKB1, promoting its ubiquitination and degradation.

Conclusions:

  • TRIM27 plays a crucial oncogenic role in glioblastoma.
  • TRIM27 contributes to GBM pathogenesis by inhibiting the LKB1/AMPK/mTOR axis, thereby enhancing the Warburg effect.
  • TRIM27 represents a potential diagnostic and therapeutic biomarker for glioblastoma.
Keywords:
AMPKGlioblastomaLKB1TRIM27Warburg effect

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