Analytical interference in CA 19-9 immunoassay generates false-positive results in a young woman with a low-grade appendiceal tumor: An educational case report and brief literature review
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View abstract on PubMed
Summary
This summary is machine-generated.A case report highlights how heterophile antibodies can cause falsely elevated CA 19-9 tumor marker levels, leading to unnecessary tests and patient distress. Using alternative methods or blocking tubes resolved this analytical interference.
Area Of Science
- Clinical Chemistry
- Laboratory Medicine
- Diagnostic Immunology
Background
- CA 19-9 (carbohydrate antigen 19-9) is a crucial tumor marker for monitoring pancreatic cancer and other digestive neoplasms.
- Elevated CA 19-9 levels in appendiceal mucocele cases can raise concerns about malignancy or rupture, necessitating careful interpretation.
Observation
- A discrepancy was noted in CA 19-9 serum concentrations between the Alinity platform and Kryptor Gold/Cobas E411 systems.
- The Alinity platform showed a persistently false elevation of CA 19-9, even post-surgery, causing patient anxiety and unnecessary medical interventions.
Findings
- Analytical interference due to heterophile antibodies was identified as the cause of the falsely elevated CA 19-9 results on the Alinity platform.
- Measurements using Kryptor Gold and Cobas E411, as well as Alinity post-neutralization with Heterophile Blocking Tubes, yielded results within the normal range.
Implications
- Analytical interferences, particularly from heterophile antibodies in immunoassays, can lead to misdiagnosis and inappropriate patient care.
- Healthcare professionals must be aware of potential interferences and employ strategies like using alternative testing methods or neutralization techniques to ensure accurate diagnostics.
- Open communication between clinicians and biologists is essential for resolving discrepancies between clinical presentation and laboratory findings.
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