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Updated: Jul 21, 2026

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
Published on: November 22, 2017
Analysis of Mitophagy Reporters in Drosophila
Alvaro Sanchez-Martinez1, Aitor Martinez1, Alexander J Whitworth2
1MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK.
Abstract:
Mitophagy is the degradation of mitochondria via the autophagy-lysosome system, disruption of which has been linked to multiple neurodegenerative diseases. As a flux process involving the identification, tagging, and degradation of subcellular components, the analysis of mitophagy benefits from the microscopy analysis of fluorescent reporters. Studying the pathogenic mechanisms of disease also benefits from analysis in animal models in order to capture the complex interplay of molecular and cell biological phenomena. Here, we describe protocols to analyze mitophagy reporters in Drosophila by light microscopy.
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