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Updated: Jun 17, 2025

Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
Proteomic Analysis of Secreted Vesicle Proteins from the Plant Cells
Haidi Yin1, Jianying Wang2, Zhong-Ping Yao3
1Multi-omics Mass Spectrometry Facility, Shenzhen Medical Academy of Research and Translation, Shenzhen, Guangdong, China.
Abstract:
A working pipeline for proteomic analysis of secreted vesicle proteins from the plant cells has been developed using urea and mass spectrometry-compatible detergent RapiGest SF, where vesicles could be efficiently lysed and membrane-bound proteins could be efficiently dissolved and digested. The vesicle lysis and the protein digestion procedures are performed within one tube to minimize the protein loss. The protein digest is analyzed using LC-MS/MS after desalting with an SPE spin column.
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