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Related Concept Videos

RNA Splicing01:32

RNA Splicing

56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Related Experiment Video

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Microglia specific alternative splicing alterations in multiple sclerosis.

Caiyun Qi1, Honglei Ren2, Yong Fan1

  • 1Department of Obstetrics and Gynecology; Guangdong Provincial Key Laboratory of Major Obstetric Diseases; Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology; Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Aging
|August 8, 2024
PubMed
Summary
This summary is machine-generated.

This study reveals cell-type specific alternative splicing (AS) changes in microglia from multiple sclerosis (MS) patients. These findings highlight novel molecular mechanisms in MS white matter pathology.

Keywords:
alternative splicingmultiple sclerosismutually exclusive exonsretained intronskipping exon

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Aberrant alternative splicing (AS) is implicated in multiple sclerosis (MS) pathogenesis.
  • Cell-type specific AS events in MS remain underexplored, particularly in microglia.

Purpose of the Study:

  • To investigate cell-type specific alternative splicing (AS) events in microglia from the white matter of MS patients.
  • To identify novel molecular pathways and potential therapeutic targets in MS.

Main Methods:

  • Utilized RNA-sequencing data from sorted CD15-CD11b+ microglia of MS patients and controls.
  • Applied rMATS to identify five types of AS events: A3SS, A5SS, SE, RI, and MXE.
  • Performed differential gene expression analysis and Gene Ontology (GO) enrichment analysis.

Main Results:

  • Identified 132 significant AS events in microglia from MS patients.
  • Skipped exon (SE) was the most prevalent AS event, followed by mutually exclusive exons (MXE) and retained introns (RI).
  • Genes involved in telomere maintenance (SE), protein folding/mitochondrion organization (MXE), and immune response (RI) showed distinct AS patterns.

Conclusions:

  • Discovered microglia-specific AS alterations in the white matter of MS patients.
  • These AS changes may contribute to MS pathogenesis.
  • Provides insights into novel pathological mechanisms and potential therapeutic strategies for MS.