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Updated: Jun 17, 2025

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
Published on: July 3, 2020
Characterizing primary transcriptional responses to short term heat shock in Down syndrome
Joseph F Cardiello1,2, Jessica Westfall3, Robin Dowell1,3,4,5
1BioFrontiers Institute, University of Colorado, Boulder, CO, United States of America.
Abstract:
Heat shock stress induces genome-wide changes in transcription regulation, activating a coordinated cellular response to enable survival. We noticed many heat shock genes are up-regulated in blood samples from individuals with trisomy 21. We characterized the immediate transcriptional response to heat shock of two lymphoblastoid cell lines derived from brothers with and without trisomy 21. The trisomy 21 cells displayed a more robust heat shock response after just one hour at 42°C than the matched disomic cells.
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