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Updated: Jun 17, 2025

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

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Published on: September 20, 2024

Evolutionary Design of Self-Templated Supramolecular Fibrils Using M13 Bacteriophage for Tissue Engineering.

Inseok Chae^1,2, Woo-Jae Chung^1,2, Hyo-Eon Jin^1,2

¹Department of Bioengineering, University of California, Berkeley, California 94720, United States.

|August 8, 2024

Summary

Researchers engineered novel biomaterials for tissue engineering using M13 bacteriophage (phage) evolved to bind hydroxyapatite (HA). These self-assembling phage materials promote dentin-like tissue regeneration, offering a promising scaffold for future applications.

Keywords:

HA mineralization M13 bacteriophage evolutionary screening self-templating assembly supramolecular fibrils tissue engineering

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Production of Nanofibrillar Patterned Collagen for Tissue Engineering

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Published on: September 20, 2024

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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

Area of Science:

Biomaterials Science
Tissue Engineering
Synthetic Biology

Background:

Nature utilizes hierarchical structures for biomaterial function across scales.
Self-assembly and binding are key natural processes for creating complex biomaterials.
Hierarchical organization is crucial for effective tissue engineering scaffolds.

Purpose of the Study:

To develop hierarchically organized tissue engineering materials inspired by nature.
To utilize evolutionary screening and self-templating assembly for material development.
To investigate the potential of M13 bacteriophage (phage) for hydroxyapatite (HA) binding and mineralization.

Main Methods:

Evolutionary screening of M13 bacteriophage against hydroxyapatite (HA) to isolate HA-binding phage (HAPh).
Characterization of HAPh length and self-assembly into supramolecular fibrils with periodic banded structures.
Assessment of HAPh fibril capability for HA mineralization and osteoblast cell growth.
Application of HAPh fibrils on dentin surfaces to evaluate dentin-like tissue regeneration.

Main Results:

Isolated HA-binding phage (HAPh) with a bimodal length (950 nm and 240 nm).
Demonstrated synergistic effect of dual lengths in forming supramolecular fibrils with periodic banded structures.
Confirmed HAPh fibrils promote HA mineralization and directional osteoblast cell growth.
Observed regeneration of dentin-like tissue structures when applied to dentin surfaces.

Conclusions:

The developed HAPh fibrils serve as effective scaffolds for dentin regeneration.
The combination of evolutionary screening and self-templating assembly is a viable strategy for creating advanced tissue engineering materials.
This approach holds significant potential for developing hierarchically organized biomaterials for regenerative medicine.