Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.5K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Liver Regeneration01:24

Liver Regeneration

3.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Fasn Promotes Anoikis Resistance In Colorectal Liver Metastases Through The Erk1/2 Pathway

FASN promotes anoikis resistance in colorectal liver metastases through the ERK1/2 pathway

Jiaru Wu1, Fei Liu2, Xudan Guo1

  • 1School of Basic Medical Sciences, Hebei University of Chinese Medicine. Shijiazhuang, Hebei, China.

Biochemical and Biophysical Research Communications
|August 8, 2024

Related Experiment Videos

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
07:59

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Published on: September 3, 2021

6.3K
Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

3.5K
Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

13.7K

View abstract on PubMed

Summary
This summary is machine-generated.

Fatty acid synthase (FASN) silencing inhibits colorectal cancer liver metastases (CRLM) by suppressing anoikis resistance (AR) via the ERK1/2 pathway. This research highlights FASN as a potential therapeutic target for CRLM.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Metastasis

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer death, with liver metastasis being common.
  • Anoikis resistance (AR) is crucial for the survival of cancer cells in metastatic sites, particularly in colorectal cancer liver metastases (CRLM).
  • Fatty acid synthase (FASN), a key enzyme in lipid synthesis, plays a role in AR in various cancers.

Purpose of the Study:

  • To investigate the role of FASN in ERK1/2-mediated AR in CRLM.
  • To evaluate the therapeutic potential of targeting FASN in CRLM.

Main Methods:

  • In vitro studies using LoVo cells to assess migration and apoptosis via scratch, migration, and flow cytometry assays.
  • Western blot analysis to quantify FASN, p-ERK1/2, and apoptosis-related proteins.
Keywords:
Anoikis resistanceColorectal liver metastasesERK1/2 pathwayFASN

Related Experiment Videos

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
07:59

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Published on: September 3, 2021

6.3K
Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

3.5K
Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

13.7K
  • Quantitative PCR (q-PCR) to measure FASN mRNA levels after silencing.
  • In vivo studies using an intrasplenic liver metastasis model in nude mice to evaluate FASN's effect on CRLM.

    • Main Results:

    • FASN silencing in vitro increased apoptosis, decreased migration, and reduced expression of anti-apoptotic proteins while increasing pro-apoptotic proteins.
    • FASN silencing also decreased p-ERK1/2 expression.
    • In vivo, FASN silencing significantly inhibited CRLM, whereas AR increased liver metastatic foci.

    Conclusions:

    • FASN silencing suppresses AR through the ERK1/2 signaling pathway.
    • Targeting FASN demonstrates therapeutic potential for inhibiting colorectal cancer liver metastasis.