Long Noncoding RNA NKX2-1-AS1 Accelerates Non-Small Cell Lung Cancer Progression through the miR-589-5p/NME1 Axis
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View abstract on PubMed
Summary
This summary is machine-generated.Long noncoding RNA NKX2-1-AS1 promotes non-small cell lung cancer (NSCLC) progression by upregulating NME1 via the miR-589-5p axis. This finding offers potential therapeutic targets for NSCLC treatment.
Area Of Science
- Molecular Oncology
- Cancer Biology
- RNA Biology
Background
- Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality globally.
- The precise mechanisms driving NSCLC progression, particularly the role of long noncoding RNAs (lncRNAs), remain incompletely understood.
- NKX2-1 antisense RNA 1 (NKX2-1-AS1) has been implicated as an oncogene in lung tumorigenesis.
Purpose Of The Study
- To elucidate the underlying mechanism of NKX2-1-AS1 in the progression of NSCLC.
- To investigate the regulatory role of NKX2-1-AS1 in NSCLC cell behavior and tumor growth.
Main Methods
- Quantitative reverse transcription PCR (RT-qPCR) for gene expression analysis.
- Cell proliferation assays (MTT, EdU), apoptosis analysis (flow cytometry), migration and invasion assays (Transwell).
- Western blot, immunofluorescence, RNA pull-down, luciferase reporter assays, and xenograft mouse models.
Main Results
- NKX2-1-AS1 expression was significantly upregulated in NSCLC tissues and cell lines.
- NKX2-1-AS1 deficiency inhibited cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while promoting apoptosis.
- NKX2-1-AS1 directly interacted with miR-589-5p, which targets NME/NM23 nucleoside diphosphate kinase 1 (NME1), thus regulating the miR-589-5p/NME1 axis and repressing tumor growth in vivo.
Conclusions
- NKX2-1-AS1 promotes NSCLC progression by upregulating NME1 through the miR-589-5p/NME1 axis.
- NKX2-1-AS1 acts as a crucial oncogenic factor in NSCLC.
- The NKX2-1-AS1/miR-589-5p/NME1 pathway represents a potential therapeutic target for NSCLC.
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